A multidimensional platform for the purification of non-coding RNA species

A renewed interest in non-coding RNA (ncRNA) has led to the discovery of novel RNA species and post-transcriptional ribonucleoside modifications, and an emerging appreciation for the role of ncRNA in RNA epigenetics. Although much can be learned by amplification-based analysis of ncRNA sequence and...

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Main Authors: McBee, Megan E. (Contributor), Su, Dan (Contributor), Pang, Yan Ling Joy (Contributor), Gu, Chen (Contributor), Prestwich, Erin (Contributor), Dedon, Peter C. (Contributor), Chionh, Yok Hian (Author), Ho, Chia-Hua (Author), Pruksakorn, Dumnoensun (Author), Indrakanti, Ramesh Babu (Contributor), Ng, Chee Sheng (Author), Hia, Fabian (Author), Dong, Hongping (Author), Shi, Pei-Yong (Author), Preiser, Peter Rainer (Author), Alonso, Sylvie (Author)
Other Authors: Massachusetts Institute of Technology. Center for Environmental Health Sciences (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
Format: Article
Language:English
Published: Oxford University Press, 2013-10-07T14:22:04Z.
Subjects:
Online Access:Get fulltext
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100 1 0 |a McBee, Megan E.  |e author 
100 1 0 |a Massachusetts Institute of Technology. Center for Environmental Health Sciences  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Department of Biological Engineering  |e contributor 
100 1 0 |a McBee, Megan E.  |e contributor 
100 1 0 |a Su, Dan  |e contributor 
100 1 0 |a Pang, Yan Ling Joy  |e contributor 
100 1 0 |a Gu, Chen  |e contributor 
100 1 0 |a Prestwich, Erin  |e contributor 
100 1 0 |a Dedon, Peter C.  |e contributor 
100 1 0 |a Indrakanti, Ramesh Babu  |e contributor 
700 1 0 |a Su, Dan  |e author 
700 1 0 |a Pang, Yan Ling Joy  |e author 
700 1 0 |a Gu, Chen  |e author 
700 1 0 |a Prestwich, Erin  |e author 
700 1 0 |a Dedon, Peter C.  |e author 
700 1 0 |a Chionh, Yok Hian  |e author 
700 1 0 |a Ho, Chia-Hua  |e author 
700 1 0 |a Pruksakorn, Dumnoensun  |e author 
700 1 0 |a Indrakanti, Ramesh Babu  |e author 
700 1 0 |a Ng, Chee Sheng  |e author 
700 1 0 |a Hia, Fabian  |e author 
700 1 0 |a Dong, Hongping  |e author 
700 1 0 |a Shi, Pei-Yong  |e author 
700 1 0 |a Preiser, Peter Rainer  |e author 
700 1 0 |a Alonso, Sylvie  |e author 
245 0 0 |a A multidimensional platform for the purification of non-coding RNA species 
260 |b Oxford University Press,   |c 2013-10-07T14:22:04Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/81340 
520 |a A renewed interest in non-coding RNA (ncRNA) has led to the discovery of novel RNA species and post-transcriptional ribonucleoside modifications, and an emerging appreciation for the role of ncRNA in RNA epigenetics. Although much can be learned by amplification-based analysis of ncRNA sequence and quantity, there is a significant need for direct analysis of RNA, which has led to numerous methods for purification of specific ncRNA molecules. However, no single method allows purification of the full range of cellular ncRNA species. To this end, we developed a multidimensional chromatographic platform to resolve, isolate and quantify all canonical ncRNAs in a single sample of cells or tissue, as well as novel ncRNA species. The applicability of the platform is demonstrated in analyses of ncRNA from bacteria, human cells and plasmodium-infected reticulocytes, as well as a viral RNA genome. Among the many potential applications of this platform are a system-level analysis of the dozens of modified ribonucleosides in ncRNA, characterization of novel long ncRNA species, enhanced detection of rare transcript variants and analysis of viral genomes. 
520 |a Singapore-MIT Alliance for Research and Technology 
520 |a National Institute of Environmental Health Sciences (ES017010) 
520 |a National Institute of Environmental Health Sciences (ES002109) 
546 |a en_US 
655 7 |a Article 
773 |t Nucleic Acids Research