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|a dc
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|a Goh, Keow Lin
|e author
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|a Massachusetts Institute of Technology. Department of Biology
|e contributor
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|a Goh, Keow Lin
|e contributor
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|a Gertler, Frank
|e contributor
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|a Cai, Li
|e author
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|a Cepko, Constance L.
|e author
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|a Gertler, Frank
|e author
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|a Ena/VASP Proteins Regulate Cortical Neuronal Positioning
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|b Elsevier,
|c 2014-01-06T14:28:58Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/83475
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|a Development of the multilayered cerebral cortex involves extensive regulated migration of neurons arising from the deeper germinative layers of the mammalian brain [1]. The anatomy and formation of the cortical layers has been well characterized; however, the underlying molecular mechanisms that control the migration and the final positioning of neurons within the cortex remain poorly understood 2 and 3. Here, we report evidence for a key role of Ena/VASP proteins, a protein family implicated in the spatial control of actin assembly [4] and previously shown to negatively regulate fibroblast cell speeds [5], in cortical development. Ena/VASP proteins are highly expressed in the developing cortical plate in cells bordering Reelin-expressing Cajal-Retzius cells and in the intermediate zone through which newly born cells migrate. Inhibition of Ena/VASP function through retroviral injections in utero led to aberrant placement of early-born pyramidal neurons in the superficial layers of both the embryonic and the postnatal cortex in a cell-autonomous fashion. The abnormally placed pyramidal neurons exhibited grossly normal morphology and polarity. Our results are consistent with a model in which Ena/VASP proteins function in vivo to control the position of neurons in the mouse neocortex.
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|a Leukemia & Lymphoma Society of America (Fellowship Award 5620-01)
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|a National Institutes of Health (U.S.) (Grant GM58801)
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|a McKnight Foundation (Scholarship Award)
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|a en_US
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|a Article
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|t Current Biology
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