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|a Han, Lin
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|a Massachusetts Institute of Technology. Center for Biomedical Engineering
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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|a Massachusetts Institute of Technology. Department of Materials Science and Engineering
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|a Massachusetts Institute of Technology. Department of Mechanical Engineering
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|a Tavakoli Nia, Hadi
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|a Ortiz, Christine
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|a Grodzinsky, Alan J.
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|a Han, Lin
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|a Li, Yang
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|a Li, Yang
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|a Ortiz, Christine
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|a Tavakoli Nia, Hadi
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|a Grodzinsky, Alan J.
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|a Poroelasticity of Cartilage at the Nanoscale
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|b Elsevier,
|c 2014-12-16T19:50:35Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/92341
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|a Atomic-force-microscopy-based oscillatory loading was used in conjunction with finite element modeling to quantify and predict the frequency-dependent mechanical properties of the superficial zone of young bovine articular cartilage at deformation amplitudes, δ, of ∼15 nm; i.e., at macromolecular length scales. Using a spherical probe tip (R ∼ 12.5 μm), the magnitude of the dynamic complex indentation modulus, |E*|, and phase angle, φ, between the force and tip displacement sinusoids, were measured in the frequency range f ∼ 0.2-130 Hz at an offset indentation depth of δ[subscript 0] ∼ 3 μm. The experimentally measured |E*| and φ corresponded well with that predicted by a fibril-reinforced poroelastic model over a three-decade frequency range. The peak frequency of phase angle, f[subscript peak], was observed to scale linearly with the inverse square of the contact distance between probe tip and cartilage, [1 over d[superscript 2]], as predicted by linear poroelasticity theory. The dynamic mechanical properties were observed to be independent of the deformation amplitude in the range δ = 7-50 nm. Hence, these results suggest that poroelasticity was the dominant mechanism underlying the frequency-dependent mechanical behavior observed at these nanoscale deformations. These findings enable ongoing investigations of the nanoscale progression of matrix pathology in tissue-level disease.
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|a National Science Foundation (U.S.) (Grant CMMI-0758651)
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|a National Institutes of Health (U.S.) (Grant AR033236)
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|a en_US
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|a Article
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|t Biophysical Journal
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