Impact of Dissociation Constant on the Detection Sensitivity of Polymerization-Based Signal Amplification Reactions
Many studies have demonstrated the concept of using free-radical polymerization reactions to provide signal amplification so that molecular recognition events indicative of disease states may be detected in a simple and low-cost manner. We provide the first systematic study of how the dissociation c...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society (ACS),
2015-02-11T20:15:20Z.
|
| Subjects: | |
| Online Access: | Get fulltext |
| Summary: | Many studies have demonstrated the concept of using free-radical polymerization reactions to provide signal amplification so that molecular recognition events indicative of disease states may be detected in a simple and low-cost manner. We provide the first systematic study of how the dissociation constant impacts detection sensitivity in these assays, having chosen a range of dissociation constants (nanomolar to picomolar) that is typical of those encountered in molecular diagnostic applications that detect protein-protein binding events. In addition, we use experimental results to validate a mass-action kinetic model that may be used to predict assay performance as an alternative or supplement to the empirical approach to developing new polymerization-based amplification assays that has characterized the field to date. National Science Foundation (U.S.). Graduate Research Fellowship Program Burroughs Wellcome Fund (Career Award at the Scientific Interface) Massachusetts Institute of Technology. James H. Ferry Fund for Innovation in Research Education Amgen Inc. |
|---|