Decreasing Herpes Simplex Viral Infectivity in Solution by Surface-Immobilized and Suspended N,N-Dodecyl,methyl-polyethylenimine

• Main Authors: Larson, Alyssa Maxine (Contributor), Oh, Hyung Suk (Author), Knipe, David M. (Author), Klibanov, Alexander M. (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
• Format: Article
• Language: English
• Published: Springer-Verlag, 2015-02-19T20:21:09Z.
• Subjects: Article
• Online Access: Get fulltext

Purpose To explore surface-immobilized and suspended modalities of the hydrophobic polycation N,N-dodecyl,methyl-polyethylenimine (DMPEI) for the ability to reduce viral infectivity in aqueous solutions containing herpes simplex viruses (HSVs) 1 and 2. Methods Surface-immobilized (coated onto surfaces) and suspended DMPEI were incubated with aqueous solutions containing HSV-1 or -2 to measure the antiviral effect of the hydrophobic polycation's formulations on HSVs. Results DMPEI coated on either polyethylene slides or male latex condoms dramatically decreases infectivity in solutions containing HSV-1 or -2. Moreover, DMPEI suspended in aqueous solution markedly reduces the infectious titer of these HSVs. Conclusion Our results suggest potential uses of DMPEI for both prophylaxis (in the form of coated condoms) and treatment (as a topical suspension) for HSV infections.
Martin Family Graduate Fellowship
National Institutes of Health (U.S.) (Grant AI057552)
United States. Army Research Office (Grant W911NF-07-D-0004)