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95472 |
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|a Rivera-Fuentes, Pablo
|e author
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|a Massachusetts Institute of Technology. Department of Chemistry
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|a Lippard, Stephen J.
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|a Rivera-Fuentes, Pablo
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|a Lippard, Stephen J.
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|a SpiroZin1: A Reversible and pH-Insensitive, Reaction-Based, Red-Fluorescent Probe for Imaging Biological Mobile Zinc
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|b Wiley Blackwell,
|c 2015-02-24T15:21:06Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/95472
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|a A reversible, reaction-based sensor for biological mobile zinc was designed, prepared, and characterized. The sensing mechanism of this probe is based on the zinc-induced, ring-opening reaction of spirobenzopyran to give a cyanine fluorophore that emits in the deep-red region of the electromagnetic spectrum. This probe is not activated by protons and operates efficiently in aqueous solution at pH 7 and high ionic strength. The mechanism of this reaction was studied by using a combination of kinetics experiments and DFT calculations. The biocompatibility of the probe was demonstrated in live HeLa cells.
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|a National Institute of General Medical Sciences (U.S.) (Grant GM 65519)
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|a Swiss National Science Foundation (Postdoctoral Fellowship)
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|a National Institutes of Health (U.S.) (1S0RR13886-01)
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|a en_US
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|a Article
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|t ChemMedChem
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