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97533 |
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|a dc
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|a Jain, Tarun
|e author
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|a Lincoln Laboratory
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|a Massachusetts Institute of Technology. Department of Mechanical Engineering
|e contributor
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|a Jain, Tarun
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|a Guerrero, Ricardo Jose S.
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|a Aguilar, Carlos A.
|e contributor
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|a Karnik, Rohit
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|a Guerrero, Ricardo Jose S.
|e author
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|a Aguilar, Carlos A.
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|a Karnik, Rohit
|e author
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|a Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes
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|b American Chemical Society (ACS),
|c 2015-06-26T13:55:34Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/97533
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|a Solid-state nanopores have emerged as versatile single-molecule sensors for applications including DNA sequencing, protein unfolding, micro-RNA detection, label-free detection of single nucleotide polymorphisms, and mapping of DNA-binding proteins involved in homologous recombination. While machining nanopores in dielectric membranes provides nanometer-scale precision, the rigid silicon support for the membrane contributes capacitive noise and limits integration with microfluidic networks for sample preprocessing. Herein, we demonstrate a technique to directly transfer solid-state nanopores machined in dielectric membranes from a silicon support into a microfluidic network. The resulting microfluidic-addressable nanopores can sense single DNA molecules at high bandwidths and with low noise, owing to significant reductions in membrane capacitance. This strategy will enable large-scale integration of solid-state nanopores with microfluidic upstream and downstream processing and permit new functions with nanopores such as complex manipulations for multidimensional analysis and parallel sensing in two and three-dimensional architectures.
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|a National Institutes of Health (U.S.) (Grant R21EB009180)
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|a United States. Air Force (Contract FA8721-05-C-0002)
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|a en_US
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|a Article
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|t Analytical Chemistry
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