Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity

Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity

No === TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP- ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and i...

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Bibliographic Details
Main Authors: Hutin, D., Grimaldi, Giulia, Matthews, J.
Language:en
Published: 2021
Subjects:
Immunoprecipitation
TCDD-inducible poly-ADP-ribose polymerase (TIPARP)
Protein purification
Biotinylated-NAD+
32P-NAD+
Online Access:http://hdl.handle.net/10454/18336
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spelling ndltd-BRADFORD-oai-bradscholars.brad.ac.uk-10454-183362021-02-12T05:01:07Z Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity Hutin, D. Grimaldi, Giulia Matthews, J. Immunoprecipitation TCDD-inducible poly-ADP-ribose polymerase (TIPARP) Protein purification Biotinylated-NAD+ 32P-NAD+ No TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP- ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins. 2021-02-10T15:04:22Z 2021-02-10T15:04:22Z 2018 2018 2018-08-11 Book chapter No full-text in the repository Hutin D, Grimaldi G and Matthews J (2018) Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity. In: Chang P (ed) ADP-ribosylation and NAD+ utilizing enzymes. Methods in Molecular Biology: 1813. Humana: New York. http://hdl.handle.net/10454/18336 en https://doi.org/10.1007/978-1-4939-8588-3_8
collection NDLTD
language en
sources NDLTD
topic Immunoprecipitation
TCDD-inducible poly-ADP-ribose polymerase (TIPARP)
Protein purification
Biotinylated-NAD+
32P-NAD+
spellingShingle Immunoprecipitation
TCDD-inducible poly-ADP-ribose polymerase (TIPARP)
Protein purification
Biotinylated-NAD+
32P-NAD+
Hutin, D.
Grimaldi, Giulia
Matthews, J.
Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
description No === TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP- ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.
author Hutin, D.
Grimaldi, Giulia
Matthews, J.
author_facet Hutin, D.
Grimaldi, Giulia
Matthews, J.
author_sort Hutin, D.
title Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
title_short Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
title_full Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
title_fullStr Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
title_full_unstemmed Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
title_sort methods to study tcdd-inducible poly-adp-ribose polymerase (tiparp) mono-adp-ribosyltransferase activity
publishDate 2021
url http://hdl.handle.net/10454/18336
work_keys_str_mv AT hutind methodstostudytcddinduciblepolyadpribosepolymerasetiparpmonoadpribosyltransferaseactivity
AT grimaldigiulia methodstostudytcddinduciblepolyadpribosepolymerasetiparpmonoadpribosyltransferaseactivity
AT matthewsj methodstostudytcddinduciblepolyadpribosepolymerasetiparpmonoadpribosyltransferaseactivity
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