Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids
no === Human saphenous vein endothelial cells (HSVECs) derived from primary cultures of adult human veins constitute an excellent in vitro model for studying human endothelial metabolism. In this study we report the14C-labelled prostanoid profile of HSVECs under resting and stimulated conditions and...
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ndltd-BRADFORD-oai-bradscholars.brad.ac.uk-10454-40382019-08-31T03:01:59Z Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids Urquhart, Paula Parkin, Susan M. Nicolaou, Anna Human saphenous vein Endothelial cells Endothelial metabolism N-3 polyunsaturated fatty acids Dietary fatty acids Eicosapentaenoic acid Docosahexaenoic acid Prostaglandin F2 ¿(PGF2 ¿). Thromboxane Calcium no Human saphenous vein endothelial cells (HSVECs) derived from primary cultures of adult human veins constitute an excellent in vitro model for studying human endothelial metabolism. In this study we report the14C-labelled prostanoid profile of HSVECs under resting and stimulated conditions and the effect of the n-3 polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid on them. Results indicate that HSVECs while under resting conditions produce mainly prostaglandin F2 ¿(PGF2 ¿). After stimulation with calcium ionophore A23187, the cells were found to synthesise PGI2, PGE2and PGF2¿as major products and thromboxane B2and PGD2as minor products. Production of14C-labelled hydroxyeicosatetraenoic acids was not detected. Eicosapentaenoic acid was found to inhibit basal and stimulated prostanoid production whereas docosahexaenoic acid inhibited basal but strongly increased stimulated prostanoid production. These results may offer the basis for further studies aiming to investigate targets for pharmacological intervention in inflammatory conditions. 2009-12-07T16:13:04Z 2009-12-07T16:13:04Z 2009-12-07T16:13:04Z Article published version paper Urquhart, P., Parkin, S. M. and Nicolaou, A. (2001). Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids. Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 65, No. 1, pp. 15-22. http://hdl.handle.net/10454/4038 en http://dx.doi.org/10.1054/plef.2001.0282 Harcourt |
collection |
NDLTD |
language |
en |
sources |
NDLTD |
topic |
Human saphenous vein Endothelial cells Endothelial metabolism N-3 polyunsaturated fatty acids Dietary fatty acids Eicosapentaenoic acid Docosahexaenoic acid Prostaglandin F2 ¿(PGF2 ¿). Thromboxane Calcium |
spellingShingle |
Human saphenous vein Endothelial cells Endothelial metabolism N-3 polyunsaturated fatty acids Dietary fatty acids Eicosapentaenoic acid Docosahexaenoic acid Prostaglandin F2 ¿(PGF2 ¿). Thromboxane Calcium Urquhart, Paula Parkin, Susan M. Nicolaou, Anna Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
description |
no === Human saphenous vein endothelial cells (HSVECs) derived from primary cultures of adult human veins constitute an excellent in vitro model for studying human endothelial metabolism. In this study we report the14C-labelled prostanoid profile of HSVECs under resting and stimulated conditions and the effect of the n-3 polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid on them. Results indicate that HSVECs while under resting conditions produce mainly prostaglandin F2 ¿(PGF2 ¿). After stimulation with calcium ionophore A23187, the cells were found to synthesise PGI2, PGE2and PGF2¿as major products and thromboxane B2and PGD2as minor products. Production of14C-labelled hydroxyeicosatetraenoic acids was not detected. Eicosapentaenoic acid was found to inhibit basal and stimulated prostanoid production whereas docosahexaenoic acid inhibited basal but strongly increased stimulated prostanoid production. These results may offer the basis for further studies aiming to investigate targets for pharmacological intervention in inflammatory conditions. |
author |
Urquhart, Paula Parkin, Susan M. Nicolaou, Anna |
author_facet |
Urquhart, Paula Parkin, Susan M. Nicolaou, Anna |
author_sort |
Urquhart, Paula |
title |
Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
title_short |
Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
title_full |
Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
title_fullStr |
Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
title_full_unstemmed |
Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
title_sort |
profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids |
publisher |
Harcourt |
publishDate |
2009 |
url |
http://hdl.handle.net/10454/4038 |
work_keys_str_mv |
AT urquhartpaula profileofeicosanoidsproducedbyhumansaphenousveinendothelialcellsandtheeffectofdietaryfattyacids AT parkinsusanm profileofeicosanoidsproducedbyhumansaphenousveinendothelialcellsandtheeffectofdietaryfattyacids AT nicolaouanna profileofeicosanoidsproducedbyhumansaphenousveinendothelialcellsandtheeffectofdietaryfattyacids |
_version_ |
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