Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry.
no === Lipidomics aim to generate qualitative and quantitative information on different classes of lipids and their species, and when applied in conjunction with proteomic and genomic assays, facilitate the comprehensive study of lipid metabolism in cellular, organ or body systems. Advances in mass...
Main Authors: | , , |
---|---|
Language: | en |
Published: |
Springer
2010
|
Subjects: | |
Online Access: | http://hdl.handle.net/10454/4575 |
id |
ndltd-BRADFORD-oai-bradscholars.brad.ac.uk-10454-4575 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-BRADFORD-oai-bradscholars.brad.ac.uk-10454-45752019-08-31T03:02:42Z Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. Nicolaou, Anna Masoodi, Mojgan Mir, Adnan A. Prostaglandins Thromboxanes Dihydro-prostaglandins Liquid Chromatography-Electrospray Ionisation-Tandem Mass Spectrometry (LC/ESI-MS/MS) Lipidomics no Lipidomics aim to generate qualitative and quantitative information on different classes of lipids and their species, and when applied in conjunction with proteomic and genomic assays, facilitate the comprehensive study of lipid metabolism in cellular, organ or body systems. Advances in mass spectrometry have underpinned the expansion of lipidomic methodologies. Prostanoids are potent autacoids present in a plethora of cellular systems, known best for their intimate role in inflammation. Electrospray ionisation (ESI) allows the efficient ionisation of prostanoids in aqueous systems. ESI can be readily coupled to liquid chromatography (LC) followed by tandem mass spectrometry (MS/MS)-based detection, thus allowing the development of a potent and selective LC/ESI-MS/MS quantitative assays. The protocol we describe in this chapter outlines the steps we follow to a) extract prostanoids from solid or liquid samples, b) semi-purify the metabolites using solid phase extraction c) set-up the HPLC separation using reverse phase chromatography and d) set up the MS/MS assay using a triple quadrupole mass spectrometer. The experimental details and notes presented here are based on the detailed protocols followed in our group 2010-12-15T10:30:27Z 2010-12-15T10:30:27Z 2009 Book chapter Nicholaou, A., Masoodi, M. and Mir, A. A. (2009). Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. In: Methods in Molecular Biology. Springer. Vol. 579, Part 2, pp. 271-286. http://hdl.handle.net/10454/4575 Methods in Molecular Biology en http://dx.doi.org/10.1007/978-1-60761-322-0_14 Springer |
collection |
NDLTD |
language |
en |
sources |
NDLTD |
topic |
Prostaglandins Thromboxanes Dihydro-prostaglandins Liquid Chromatography-Electrospray Ionisation-Tandem Mass Spectrometry (LC/ESI-MS/MS) Lipidomics |
spellingShingle |
Prostaglandins Thromboxanes Dihydro-prostaglandins Liquid Chromatography-Electrospray Ionisation-Tandem Mass Spectrometry (LC/ESI-MS/MS) Lipidomics Nicolaou, Anna Masoodi, Mojgan Mir, Adnan A. Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
description |
no === Lipidomics aim to generate qualitative and quantitative information on different classes of lipids and their species, and when applied in conjunction with proteomic and genomic assays, facilitate the comprehensive study of lipid metabolism in cellular, organ or body systems. Advances in mass spectrometry have underpinned the expansion of lipidomic methodologies. Prostanoids are potent autacoids present in a plethora of cellular systems, known best for their intimate role in inflammation. Electrospray ionisation (ESI) allows the efficient ionisation of prostanoids in aqueous systems. ESI can be readily coupled to liquid chromatography (LC) followed by tandem mass spectrometry (MS/MS)-based detection, thus allowing the development of a potent and selective LC/ESI-MS/MS quantitative assays. The protocol we describe in this chapter outlines the steps we follow to a) extract prostanoids from solid or liquid samples, b) semi-purify the metabolites using solid phase extraction c) set-up the HPLC separation using reverse phase chromatography and d) set up the MS/MS assay using a triple quadrupole mass spectrometer. The experimental details and notes presented here are based on the detailed protocols followed in our group |
author |
Nicolaou, Anna Masoodi, Mojgan Mir, Adnan A. |
author_facet |
Nicolaou, Anna Masoodi, Mojgan Mir, Adnan A. |
author_sort |
Nicolaou, Anna |
title |
Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
title_short |
Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
title_full |
Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
title_fullStr |
Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
title_full_unstemmed |
Lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
title_sort |
lipidomic analysis of prostanoids by liquid chromatography-electrospray tandem mass spectrometry. |
publisher |
Springer |
publishDate |
2010 |
url |
http://hdl.handle.net/10454/4575 |
work_keys_str_mv |
AT nicolaouanna lipidomicanalysisofprostanoidsbyliquidchromatographyelectrospraytandemmassspectrometry AT masoodimojgan lipidomicanalysisofprostanoidsbyliquidchromatographyelectrospraytandemmassspectrometry AT miradnana lipidomicanalysisofprostanoidsbyliquidchromatographyelectrospraytandemmassspectrometry |
_version_ |
1719239722027450368 |