Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry.
no === Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) and exhibit a range of tissue and cell-specific activities in many physiological and pathological processes. Electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatil...
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ndltd-BRADFORD-oai-bradscholars.brad.ac.uk-10454-45822019-08-31T03:02:42Z Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. Masoodi, Mojgan Mir, Adnan A. Petasis, N.A. Serhan, S.N. Nicolaou, Anna Hydroxy fatty acids Eicosanoids Arachidonic Acid Eicosapentaenoic Acid Resolvins Protectin Docosahexaenoic acid Polyunsaturated fatty acids (PUFA) Liquid Chromatograpy Electrospray Ionisation Tandem Mass Spectrometry (LC/ESI-MS/MS) Lipid mediators no Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) and exhibit a range of tissue and cell-specific activities in many physiological and pathological processes. Electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI-MS/MS assay for the simultaneous analysis of twenty mono- and poly-hydroxy fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1-100 pg/¿L, whilst the limits of detection and quantitation were 10-20 and 20-50 pg respectively. The recovery of the extraction methodology varied from 76-122% depending on the metabolite. This system is useful for profiling a range of biochemically-related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxy-eicosapentaenoic and hydroxy-docosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease. Wellcome Trust, British Heart Foundation 2010-12-15T16:03:09Z 2010-12-15T16:03:09Z 2008 Article Accepted Manuscript Masoodi, M., Mir, A. A., Petasis, N. A., Serhan, C. N. and Nicolaou, A. (2008). Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry, Vol. 22, No 2., pp.75¿83. http://hdl.handle.net/10454/4582 Rapid Communications in Mass Spectrometry en http://dx.doi.org/10.1002/rcm.3331 Wiley |
collection |
NDLTD |
language |
en |
sources |
NDLTD |
topic |
Hydroxy fatty acids Eicosanoids Arachidonic Acid Eicosapentaenoic Acid Resolvins Protectin Docosahexaenoic acid Polyunsaturated fatty acids (PUFA) Liquid Chromatograpy Electrospray Ionisation Tandem Mass Spectrometry (LC/ESI-MS/MS) Lipid mediators |
spellingShingle |
Hydroxy fatty acids Eicosanoids Arachidonic Acid Eicosapentaenoic Acid Resolvins Protectin Docosahexaenoic acid Polyunsaturated fatty acids (PUFA) Liquid Chromatograpy Electrospray Ionisation Tandem Mass Spectrometry (LC/ESI-MS/MS) Lipid mediators Masoodi, Mojgan Mir, Adnan A. Petasis, N.A. Serhan, S.N. Nicolaou, Anna Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
description |
no === Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) and exhibit a range of tissue and cell-specific activities in many physiological and pathological processes. Electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI-MS/MS assay for the simultaneous analysis of twenty mono- and poly-hydroxy fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1-100 pg/¿L, whilst the limits of detection and quantitation were 10-20 and 20-50 pg respectively. The recovery of the extraction methodology varied from 76-122% depending on the metabolite. This system is useful for profiling a range of biochemically-related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxy-eicosapentaenoic and hydroxy-docosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease. === Wellcome Trust, British Heart Foundation |
author |
Masoodi, Mojgan Mir, Adnan A. Petasis, N.A. Serhan, S.N. Nicolaou, Anna |
author_facet |
Masoodi, Mojgan Mir, Adnan A. Petasis, N.A. Serhan, S.N. Nicolaou, Anna |
author_sort |
Masoodi, Mojgan |
title |
Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
title_short |
Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
title_full |
Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
title_fullStr |
Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
title_full_unstemmed |
Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
title_sort |
simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. |
publisher |
Wiley |
publishDate |
2010 |
url |
http://hdl.handle.net/10454/4582 |
work_keys_str_mv |
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