Biochemistry and Genetics of Canavanine Resistance in Neurospora

• Main Author: Logan, James Barrie
• Format: Others
• Language: en
• Published: 1969
• Online Access: https://thesis.library.caltech.edu/10251/1/Logan_JB_1969.pdf; Logan, James Barrie (1969) Biochemistry and Genetics of Canavanine Resistance in Neurospora. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/2j3p-es09. https://resolver.caltech.edu/CaltechTHESIS:06022017-085722120 <https://resolver.caltech.edu/CaltechTHESIS:06022017-085722120>

<p>The pattern of inheritance of resistance to growth inhibition by canavanine in Neurospora crassa is shown to result from interactions between a major gene and several modifiers. The major gene controls the production of a constitutive enzyme that destroys canavanine. The modifiers affect the rate of uptake of the analog from the medium. Strains which lack the enzyme activity are canavanine sensitive; strains which possess it are resistant, but the level of resistance is dependent on the rate of uptake.</p> <p>The canavanine degrading enzyme was partially purified and its properties studied. The detoxification reaction was shown to be a cleavage of canavanine yielding hydroxyguanidine.</p>