Surface markers of regionalization in the vertebrate nervous system
<p>In order to identify new molecules that might play a role in regional specification of the nervous system, we generated and characterized monoclonal antibodies (mAbs) that have positionally-restricted labeling patterns.</p> <p>The FORSE-1 mAb was generated using a strategy...
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Format: | Others |
Language: | en |
Published: |
1994
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Online Access: | https://thesis.library.caltech.edu/7720/1/Tole_s_1994.pdf Tole, Shubha (1994) Surface markers of regionalization in the vertebrate nervous system. Dissertation (Ph.D.), California Institute of Technology. https://resolver.caltech.edu/CaltechTHESIS:05172013-074622662 <https://resolver.caltech.edu/CaltechTHESIS:05172013-074622662> |
Summary: | <p>In order to identify new molecules that might play a role in regional
specification of the nervous system, we generated and characterized
monoclonal antibodies (mAbs) that have positionally-restricted labeling
patterns.</p>
<p>The FORSE-1 mAb was generated using a strategy designed to produce
mAbs against neuronal cell surface antigens that might be regulated by
regionally-restricted transcription factors in the developing central nervous
system (CNS). FORSE-1 staining is enriched in the forebrain as compared to
the rest of the CNS until E18. Between E11.5-E13.5, only certain areas of the
forebrain are labeled. There is also a dorsoventrally-restricted region of
labeling in the hindbrain and spinal cord. The mAb labels a large
proteoglycan-like cell-surface antigen (>200 kD). The labeling pattern of
FORSE-1 is conserved in various mammals and in chick.</p>
<p>To determine whether the FORSE-1 labeling pattern is similar to that
of known transcription factors, the expression of BF-1 and Dlx-2 was
compared with FORSE-1. There is a striking overlap between BF-1 and
FORSE-1 in the telencephalon. In contrast, FORSE-1 and Dlx-2 have very
different patterns of expression in the forebrain, suggesting that regulation by
Dlx-2 alone cannot explain the distribution of FORSE-1. They do, however,
share some sharp boundaries in the diencephalon. In addition, FORSE-1
identifies some previously unknown boundaries in the developing forebrain.
Thus, FORSE-1 is a new cell surface marker that can be used to subdivide the
embryonic forebrain into regions smaller than previously described,
providing further complexity necessary for developmental patterning.</p>
<p>I also studied the expression of the cell surface protein CD9 in the
developing and adult rat nervous system. CD9 is implicated in intercellular
signaling and cell adhesion in the hematopoetic system. In the nervous
system, CD9 may perform similar functions in early sympathetic ganglia,
chromaffin cells, and motor neurons, all of which express the protein. The
presence of CD9 on the surfaces of Schwann cells and axons at the appropriate
time may allow the protein to participate in the cellular interactions involved
in myelination.</p> |
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