Prédiction des séquences cis-regulatrices tissu-spécifiques: application à l'ascidie Ciona intestinalis et au neurectoderme antérieur
The detection and annotation of cis-regulatory sequences is a difficult problem. There is currently no generally applicable experimental procedure or computational algorithm to identify the non-coding regions of the genome that serve to activate gene expression in a given cell type. The only indicat...
| Main Author: | |
|---|---|
| Language: | ENG |
| Published: |
Université Paris Sud - Paris XI
2009
|
| Subjects: | |
| Online Access: | http://tel.archives-ouvertes.fr/tel-00413501 http://tel.archives-ouvertes.fr/docs/00/41/35/01/PDF/thesisHaeussler.pdf |
| Summary: | The detection and annotation of cis-regulatory sequences is a difficult problem. There is currently no generally applicable experimental procedure or computational algorithm to identify the non-coding regions of the genome that serve to activate gene expression in a given cell type. The only indicator of cis-regulatory function is the conservation of a sequence in other genomes. Regions can then be tested one-by-one in transgenic assays but this is time-consuming in vertebrates. Only a limited number of these already validated cis-regulatory sequences have been curated in biological databases. One of the main advantages of the model organism Ciona intestinalis is that cis-regulatory tests can be conducted very easily and the result is observable after one day while the animal follows the chordate body plan. However, a sequence found to be active in this organism can currently not be mapped to genomes of other animals. |
|---|