Autophagic degradation of peroxisomes in the alkane-assimilating yeast Yarrowia lipolytica

• Main Author: Parshyna, Iryna
• Other Authors: Technische Universität Dresden, Biologie
• Format: Doctoral Thesis
• Language: English
• Published: Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden 2006
• Subjects: Peroxisome; pexophagy; autophagy; Yarrowia lipolytica; pdd mutant; Peroxisom; Pexophagie; Autophagie; ddc:570; rvk:WF 9500; Autophagie <Physiologie>
• Online Access: http://nbn-resolving.de/urn:nbn:de:swb:14-1164836301920-35300; http://nbn-resolving.de/urn:nbn:de:swb:14-1164836301920-35300; http://www.qucosa.de/fileadmin/data/qucosa/documents/1650/1164836301920-3530.pdf

The thesis is aimed at understanding of molecular mechanisms of autophagic degradation of peroxisomes (pexophagy) in the yeast Yarrowia lipolytica. This microorganism has been extensively used to explore peroxisome biogenesis (Titorenko and Rachubinski, 2000). Gunkel et al. (1999) intoduced Y. lypolitica into pexophagy studies. However, the field of pexophagic research on this yeast remains quite unexplored. This work involved following tasks: (1) the development and optimization of Y. lipolytica as a model system to study peroxisome degradation; (2) Y. lipolytica genes and proteins implicated in pexophagy should be found and characterized; (3) a proper easy-to-handle selection procedure to isolate novel peroxisome degradation-deficient(pdd) mutants of Y. lipolytica should be devised.