NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS
N-Acylethanolamines (NAEs), although are ubiquitous in plants and animals the occurrence of endocannabinoid ligands and the corresponding cannabinoid receptors was limited to mammals. Interestingly, bryophytes, unlike seed plants possess arachidonic acid (AA, 20:4) and eicosapentaenoic acid (EPA, 20...
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ndltd-ETSU-oai-dc.etsu.edu-asrf-10792019-05-16T05:13:09Z NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS Shinde, Suhas Welti, Ruth Kilaru, Aruna N-Acylethanolamines (NAEs), although are ubiquitous in plants and animals the occurrence of endocannabinoid ligands and the corresponding cannabinoid receptors was limited to mammals. Interestingly, bryophytes, unlike seed plants possess arachidonic acid (AA, 20:4) and eicosapentaenoic acid (EPA, 20:5), which are fatty acid precursors for endocannabinoid ligands. Here, we show that the moss Physcomitrella patens contains ~24 and 7 % of AA and EPA, respectively. Using selective lipidomic profiling, we identified polyunsaturated NAEs, including N-arachidonoyl ethanolamide (anandamide/AEA/NAE 20:4) and N-eicosapentaenoyl ethanolamide (EPEA) and also their corresponding N-acyl-phosphatidylethanolamine (NAPE) precursors in various developmental stages of Physcomitrella. Quantification of various NAPE and NAE species indicated the abundance of unsaturated species over saturated. In all haploid developmental stages analyzed, NAE 20:4 levels contributed to ~ 30 % (~ 26 ng mg-1 lipid) of the total NAE while NAE 20:5 remained as a minor component (~ 5 %; ~ 4.5 ng mg-1 lipid). Exogenous application of AEA, EPEA and their corresponding fatty acid precursors (AA and EPA, respectively) inhibited the growth of gametophytes and protonemata in a dose-dependent manner. AEA has shown the exclusive effect on the F-actin dynamics at the apex of protonemal cells, which was similar to the effect of abscisic acid (ABA) on protonemal growth inhibition. Additionally, we identified moss ortholog for NAPE-hydrolyzing phospholipase D (NAPE-PLD) enzyme that was responsive to exogenous ABA. Putative PpNAPE-PLD was expressed in E. coli for further characterization. Our data demonstrate the occurrence of evolutionarily conserved NAE metabolic pathway in the moss, with the occurrence of AEA and EPEA. 2018-04-04T20:40:00Z text application/pdf https://dc.etsu.edu/asrf/2018/schedule/111 Appalachian Student Research Forum Digital Commons @ East Tennessee State University N-Acylethanolamines anandamide N-eicosapentaenoyl ethanolamide Physcomitrella patens Biochemistry Molecular Biology |
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N-Acylethanolamines anandamide N-eicosapentaenoyl ethanolamide Physcomitrella patens Biochemistry Molecular Biology |
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N-Acylethanolamines anandamide N-eicosapentaenoyl ethanolamide Physcomitrella patens Biochemistry Molecular Biology Shinde, Suhas Welti, Ruth Kilaru, Aruna NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS |
description |
N-Acylethanolamines (NAEs), although are ubiquitous in plants and animals the occurrence of endocannabinoid ligands and the corresponding cannabinoid receptors was limited to mammals. Interestingly, bryophytes, unlike seed plants possess arachidonic acid (AA, 20:4) and eicosapentaenoic acid (EPA, 20:5), which are fatty acid precursors for endocannabinoid ligands. Here, we show that the moss Physcomitrella patens contains ~24 and 7 % of AA and EPA, respectively. Using selective lipidomic profiling, we identified polyunsaturated NAEs, including N-arachidonoyl ethanolamide (anandamide/AEA/NAE 20:4) and N-eicosapentaenoyl ethanolamide (EPEA) and also their corresponding N-acyl-phosphatidylethanolamine (NAPE) precursors in various developmental stages of Physcomitrella. Quantification of various NAPE and NAE species indicated the abundance of unsaturated species over saturated. In all haploid developmental stages analyzed, NAE 20:4 levels contributed to ~ 30 % (~ 26 ng mg-1 lipid) of the total NAE while NAE 20:5 remained as a minor component (~ 5 %; ~ 4.5 ng mg-1 lipid). Exogenous application of AEA, EPEA and their corresponding fatty acid precursors (AA and EPA, respectively) inhibited the growth of gametophytes and protonemata in a dose-dependent manner. AEA has shown the exclusive effect on the F-actin dynamics at the apex of protonemal cells, which was similar to the effect of abscisic acid (ABA) on protonemal growth inhibition. Additionally, we identified moss ortholog for NAPE-hydrolyzing phospholipase D (NAPE-PLD) enzyme that was responsive to exogenous ABA. Putative PpNAPE-PLD was expressed in E. coli for further characterization. Our data demonstrate the occurrence of evolutionarily conserved NAE metabolic pathway in the moss, with the occurrence of AEA and EPEA. |
author |
Shinde, Suhas Welti, Ruth Kilaru, Aruna |
author_facet |
Shinde, Suhas Welti, Ruth Kilaru, Aruna |
author_sort |
Shinde, Suhas |
title |
NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS |
title_short |
NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS |
title_full |
NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS |
title_fullStr |
NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS |
title_full_unstemmed |
NOVEL POLYUNSATURATED N-ACYLETHANOLAMINES AND THEIR IMPLICATIONS IN PHYSCOMITRELLA PATENS |
title_sort |
novel polyunsaturated n-acylethanolamines and their implications in physcomitrella patens |
publisher |
Digital Commons @ East Tennessee State University |
publishDate |
2018 |
url |
https://dc.etsu.edu/asrf/2018/schedule/111 |
work_keys_str_mv |
AT shindesuhas novelpolyunsaturatednacylethanolaminesandtheirimplicationsinphyscomitrellapatens AT weltiruth novelpolyunsaturatednacylethanolaminesandtheirimplicationsinphyscomitrellapatens AT kilaruaruna novelpolyunsaturatednacylethanolaminesandtheirimplicationsinphyscomitrellapatens |
_version_ |
1719189571573383168 |