Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917.
Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric...
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ndltd-ETSU-oai-dc.etsu.edu-etd-33532019-05-16T04:45:52Z Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. Hammond, David Jack Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric ions, bind with high affinity, and transport it into the cell via multicomponent transport systems. Rhizobia are soil dwelling organisms that form symbiotic relationships with host plants and fix atmospheric nitrogen, while the bacteria receive nutrients. R. leguminosarum IARI 917 produces a siderophore characterized as 'schizokinen'. In the present study, we have characterized the binding and transport kinetics of 'schizokinen' and have also attempted to identify the genes involved in its biosynthesis using mini Tn5 random mutagenesis. DNA sequence analysis of a non siderophore producing transconjugant revealed a gene involved in PAC/PAS signal transduction systems that respond to many extracellular cues. 2008-12-13T08:00:00Z text application/pdf https://dc.etsu.edu/etd/2001 https://dc.etsu.edu/cgi/viewcontent.cgi?article=3353&context=etd Copyright by the authors. Electronic Theses and Dissertations Digital Commons @ East Tennessee State University Rhizobia siderophore schizokinen Chemical Actions and Uses Chemicals and Drugs Medicine and Health Sciences |
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Rhizobia siderophore schizokinen Chemical Actions and Uses Chemicals and Drugs Medicine and Health Sciences |
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Rhizobia siderophore schizokinen Chemical Actions and Uses Chemicals and Drugs Medicine and Health Sciences Hammond, David Jack Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. |
description |
Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric ions, bind with high affinity, and transport it into the cell via multicomponent transport systems. Rhizobia are soil dwelling organisms that form symbiotic relationships with host plants and fix atmospheric nitrogen, while the bacteria receive nutrients. R. leguminosarum IARI 917 produces a siderophore characterized as 'schizokinen'. In the present study, we have characterized the binding and transport kinetics of 'schizokinen' and have also attempted to identify the genes involved in its biosynthesis using mini Tn5 random mutagenesis. DNA sequence analysis of a non siderophore producing transconjugant revealed a gene involved in PAC/PAS signal transduction systems that respond to many extracellular cues. |
author |
Hammond, David Jack |
author_facet |
Hammond, David Jack |
author_sort |
Hammond, David Jack |
title |
Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. |
title_short |
Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. |
title_full |
Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. |
title_fullStr |
Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. |
title_full_unstemmed |
Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917. |
title_sort |
characterization of the genes involved in biosynthesis and transport of schizokinen, a siderophore produced by <em>rhizobium leguminosarum</em> iari 917. |
publisher |
Digital Commons @ East Tennessee State University |
publishDate |
2008 |
url |
https://dc.etsu.edu/etd/2001 https://dc.etsu.edu/cgi/viewcontent.cgi?article=3353&context=etd |
work_keys_str_mv |
AT hammonddavidjack characterizationofthegenesinvolvedinbiosynthesisandtransportofschizokinenasiderophoreproducedbyemrhizobiumleguminosarumemiari917 |
_version_ |
1719188157524606976 |