Mutational Analysis of Substrate Specificity in a Citrus Paradisi Flavonol 3- O-Glucosyltransferase

Mutational Analysis of Substrate Specificity in a Citrus Paradisi Flavonol 3- O-Glucosyltransferase

Citrus paradisi 3-O-glucosyltransferase (Cp3GT, Genbank Protein ID: ACS15351) and Citrus sinensis 3-O-glucosyltransferase (Cs3GT, Genbank Protein ID: AAS00612.2) share 95% amino acid sequence identity. Cp3GT was previously established as a flavonol-specific 3-O-glucosyltransferase by direct enzymati...

Full description

Bibliographic Details
Main Authors:	Devaiah, Shivakumar P., Tolliver, Benjamin M., Zhang, Cheng, Owens, Daniel K., McIntosh, Cecilia A.
Published:	Digital Commons @ East Tennessee State University 2018
Subjects:	citrus paradisi citrus sinensis flavonoid glucosyltransferase site-directed mutagenesis substrate specificity Biological Sciences Biochemistry Molecular Biology Plant Biology
Online Access:	https://dc.etsu.edu/etsu-works/2742 https://doi.org/10.1007/s13562-017-0411-0

Similar Items

Towards Understanding of Glucosyltransferase Specifi city in Citrus Paradisi
by: Devaiah, Shivakumar P., et al.
Published: (2013)

Substrate Specificity and Kinetic Properties of Flavonol-3-O-Glucosyltransferase From Citrus Paradisi
by: Devaiah, Shivakumar P., et al.
Published: (2013)

Determination of the Substrate Specificity of the Mutant D344P of Citrus paradisi Flavonol-Specific 3-O-Glucosyltransferase
by: Spaulding, Nathan, et al.
Published: (2017)

Site-Directed Mutagenesis in Citrus paradisi Flavonol-Specific 3-O-Glucosyltransferase
by: Khaja, Sara
Published: (2014)

Site-Directed Mutational Analysis of Flavonol 3-0-Glucosyltransferases from Citrus paradisi
by: Devaiah, Shivakumar P., et al.
Published: (2013)

Effect of Mutant P145T on the Enzyme Activity of Glucosyltransferase from Citrus paradisi
by: Kandel, Sangam, et al.
Published: (2015)

Effects of Amino Acid Insertion on the Substrate and Regiospecificity of a Citrus paradisi Glucosyltransferase
by: Tolliver, Benjamin M., et al.
Published: (2014)

Expression and Biochemical Characterization of Two Glucosyltransferases from Citrus paradisi
by: Devaiah, Shivakumar P., et al.
Published: (2012)

Structure and Functional Analysis of Glucosyltransferase from Citrus paradisi
by: Devaiah, Shivakumar P., et al.
Published: (2014)

Cloning, Expression, and Biochemical Characterization of Recombinant Putative Glucosyltransferases Clone 3 and 8 from Grapefruit (Citrus paradisi)
by: Hayford, Deborah
Published: (2012)

The Effect of R382W Mutation on C. paradisi Flavonol-Specific 3-O-Glucosyltransferase
by: King, Kathleen, et al.
Published: (2015)

Effects of Amino Acid Sequence Insertion on the Substrate Preference of a Citrus Paradisi Glucosyltransferase
by: Tolliver, Benjamin M., et al.
Published: (2013)

Putative Glucosyltransferase 11 from Citrus paradisi: Cloning, Recombinant Expression in Yeast, and Substrate Screening
by: Williams, Bruce E., et al.
Published: (2013)

Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)
by: Wamucho, Anye
Published: (2012)

Investigating Potentially Key Residues Which Imparts the Substrate and Regiospecifi city of aFlavonol-Specifi c 3-O-Glucosyltransferase from Grapefruit
by: Adepoju, Olusegun A., et al.
Published: (2013)

Selected Point Mutations of a Flavonoid 3-O-Glucosyltransferase from Citrus paradisi (Grapefruit) and Effect on Substrate and Regiospecificity
by: Adepoju, Olusegun A., et al.
Published: (2013)

Structure-Function Investigations of Site-Directed Mutants of Citrus paradisi Flavonol-Specific 3 O Glucosyltransferase (Cp3OGT) – Impact of Mutations of Serine, Histidine, and Glutamine
by: Sathanantham, Preethi, et al.
Published: (2015)

Selected Point Mutation of a Flavonoid 3-0-Glucosyltransferase from Citrus paradisi (Grapefruit) and its Effect on Substrate and Regiospecificity
by: Adepoju, Olusegun A., et al.
Published: (2013)

Analysis of Impact of R382W Mutation on Substrate Specificity of Grapefruit Flavonol Specific 3-Glucosyltransferase
by: King, Kathleen, et al.
Published: (2015)

Using Site-Directed Mutagenesis to Determine Impact of Amino Acid Substitution on Substrate and Regiospecificity of Grapefruit Flavonol 3-O-Glucosyltransferase
by: Adepoju, Olusegun A., et al.
Published: (2014)

Expression and Characterization of Putative Plant Secondary Product Glucosyltransferase Clone 10 from <em>Citrus paradisi</em> and Resolution of Multiple Bands in PGT5/6.
by: Lin, Zhangfan
Published: (2011)

Structure and Function of Flavonoid Glucosyltransferases: Using a Specific Grapefruit Enzyme as a Model
by: McIntosh, Cecilia A.
Published: (2015)

Structure and Function of Flavonoid Glucosyltransferases: Using a specific Grapefruit Enzyme as a Model
by: McIntosh, Cecilia A.
Published: (2016)

Mutagenesis of a Flavonol- 3-O-Glucosyltransferase and the Effect on Enzyme Function
by: Carter, Lisa, et al.
Published: (2013)

Crystallization of a Flavonol-Specific 3-O-Glucosyltrasnferase found in Citrus paradisi
by: Birchfield, Aaron, et al.
Published: (2017)

Heterologous Expression and Characterization of Putative Secondary Product Glucosyltransferase (PGT)Clones 4 and 11 Isolated from Citrus paradisi
by: Loftis, Peri, et al.
Published: (2013)

Position-Specific Flavonoid Glucosyltransferases: Structure and Functional Analysis of Grapefruit Flavonol-Specific 3-O-GT
by: McIntosh, Cecilia A.
Published: (2014)

Structure-Function Analysis of Grapefruit Glucosyltransferase Protein – Identification of Key Amino Acid Residues for its Rigid Substrate Specificity
by: Sathanantham, Preethi, et al.
Published: (2015)

Biochemical Characterization of a Cp-3-O-GT Mutant P145T and Study of the Tag Effect on GT Activity
by: Kandel, Sangam, et al.
Published: (2016)

Recombination and Screening of Putative Glucosyltransferase Clone 4 in Pichia pastoris
by: Loftis, Peri, et al.
Published: (2012)

Recombination and Screening of Putative Grapefruit Glucosyltransferase 4 in Pichia pastoris
by: Loftis, Peri, et al.
Published: (2013)

Structural and Functional Analysis of Grapefruit Flavonol-Specific-3-O-GT Mutant P145T
by: Kandel, Sangam, Mr
Published: (2016)

Modification, Verification of Sequence and Optimization of Expression of P297F an Inactive Mutant of Flavonol Specific Glucosyltransferase from Grapefruit (CP3GT)
by: Fox, Sarah
Published: (2020)

The Effect of Recombinant Tags on <i>Citrus paradisi</i> Flavonol-Specific 3-O Glucosyltransferase Activity
by: Aaron S. Birchfield, et al.
Published: (2020-03-01)

Determining Putative Secondary Product Glucosyltransferase Expression During <em>Citrus paradisi</em> Growth and Development.
by: Daniel, Jala
Published: (2009)

Crystallization of a Flavonol-Specific 3-O Glucosyltransferase and Site-Directed Mutants from Grapefruit
by: Birchfield, Aaron, et al.
Published: (2019)

EFEITO DA COMPOSIÇÃO DO SUBSTRATO NA FORMAÇÃO DE MUDAS DE LARANJEIRA `PERA'
by: F.A.A. MOURÃO FILHO, et al.
Published: (1998-01-01)

Determination of the Substrate Specificity of Citrus paradisi Flavonol Specific 3-O-Glucosyltransferase Mutant D344P
by: Spaulding, Nathan R
Published: (2017)

cDNA Cloning, Expression and Characterization of a Putative Glucosyltransferase (GT) from Grapefruit (<em>Citrus paradisi</em>) Leaves.
by: Roy Sarkar, Tapasree
Published: (2004)

Chemical Compositions of the Peel Essential Oil of Citrus aurantium and its Natural Larvicidal Activity against the Malaria Vector Anopheles stephensi (Diptera: Culicidae) in Comparison with Citrus paradisi
by: Alireza Sanei-Dehkordi, et al.
Published: (2016-10-01)

Cannot write session to /tmp/vufind_sessions/sess_qjkg2ms8k3segeviv09tng08ar