Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine

A basic understanding of the molecular events occurring during zygotic embryogenesis is required to fully understand how and why only a very small percentage of somatic embryos develop past the late embryogeny phase of embryogenesis. In this work, we have identified genes that have been demonstrate...

Full description

Bibliographic Details
Main Author:	Jones, Brande
Published:	Georgia Institute of Technology 2012
Subjects:	Plant genetics Somatic embryogenesis Root development Arabidopsis Arabidopsis thaliana Loblolly pine Roots (Botany) Development
Online Access:	http://hdl.handle.net/1853/43598

id	ndltd-GATECH-oai-smartech.gatech.edu-1853-43598
record_format	oai_dc
spelling	ndltd-GATECH-oai-smartech.gatech.edu-1853-435982013-01-07T20:38:50ZIdentification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pineJones, BrandePlant geneticsSomatic embryogenesisRoot developmentArabidopsisArabidopsis thalianaLoblolly pineRoots (Botany) DevelopmentA basic understanding of the molecular events occurring during zygotic embryogenesis is required to fully understand how and why only a very small percentage of somatic embryos develop past the late embryogeny phase of embryogenesis. In this work, we have identified genes that have been demonstrated to be required for late embryonic development in the model plant system Arabidopsis thaliana. These genes were subsequently isolated and cloned from Loblolly pine embryos. These isolated clones were sequenced and analyzed to reveal significant homology to the known Arabidopsis ABA responsive genes ABI3, ABI4, and ABI5. Expression analyses of all three genes were completed, and compared to reported data of ABA accumulation, as well as, expression of other ABA responsive genes during the same stages of embryogenesis. Six putative root development genes were isolated and cloned from Loblolly pine embryos. These isolated clones were sequenced and analyzed to reveal significant homology to the known Arabidopsis root development genes WOODENLEG, SHORT ROOT, SCARECROW, HOBBIT, BODENLOS, and MONOPTEROS. Full-length cDNAs were isolated and cloned for WOODENLEG, SHORT ROOT, SCARECROW and BODENLOS. Expression analyses of all six genes were completed throughout mid to late embryogenesis in Loblolly pine.Georgia Institute of Technology2012-06-06T16:42:57Z2012-06-06T16:42:57Z2011-01-22Dissertationhttp://hdl.handle.net/1853/43598
collection	NDLTD
sources	NDLTD
topic	Plant genetics Somatic embryogenesis Root development Arabidopsis Arabidopsis thaliana Loblolly pine Roots (Botany) Development
spellingShingle	Plant genetics Somatic embryogenesis Root development Arabidopsis Arabidopsis thaliana Loblolly pine Roots (Botany) Development Jones, Brande Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
description	A basic understanding of the molecular events occurring during zygotic embryogenesis is required to fully understand how and why only a very small percentage of somatic embryos develop past the late embryogeny phase of embryogenesis. In this work, we have identified genes that have been demonstrated to be required for late embryonic development in the model plant system Arabidopsis thaliana. These genes were subsequently isolated and cloned from Loblolly pine embryos. These isolated clones were sequenced and analyzed to reveal significant homology to the known Arabidopsis ABA responsive genes ABI3, ABI4, and ABI5. Expression analyses of all three genes were completed, and compared to reported data of ABA accumulation, as well as, expression of other ABA responsive genes during the same stages of embryogenesis. Six putative root development genes were isolated and cloned from Loblolly pine embryos. These isolated clones were sequenced and analyzed to reveal significant homology to the known Arabidopsis root development genes WOODENLEG, SHORT ROOT, SCARECROW, HOBBIT, BODENLOS, and MONOPTEROS. Full-length cDNAs were isolated and cloned for WOODENLEG, SHORT ROOT, SCARECROW and BODENLOS. Expression analyses of all six genes were completed throughout mid to late embryogenesis in Loblolly pine.
author	Jones, Brande
author_facet	Jones, Brande
author_sort	Jones, Brande
title	Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
title_short	Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
title_full	Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
title_fullStr	Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
title_full_unstemmed	Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
title_sort	identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in loblolly pine
publisher	Georgia Institute of Technology
publishDate	2012
url	http://hdl.handle.net/1853/43598
work_keys_str_mv	AT jonesbrande identificationisolationexpressionanalysisandmolecularcharacterizationofninegeneskeytolateembryogenesisinloblollypine
_version_	1716475694222934016

Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine

Similar Items