Avalia??o de produtos naturais no controle de biofilmes formados em sistema din?mico

• Main Author: Viana, Marcelino Gevilbergue
• Other Authors: CPF:13893734449
• Format: Others
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2014
• Subjects: Biofilme; Biocorros?o; Lippia gracilis Schauer; Biocida e corros?o; Biofilm; Biocorrosion; Biocid and corrosion; CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::TECNOLOGIA QUIMICA::PETROLEO E PETROQUIMICA
• Online Access: http://repositorio.ufrn.br:8080/jspui/handle/123456789/12903

Made available in DSpace on 2014-12-17T14:08:35Z (GMT). No. of bitstreams: 1 MarcelinoGVpdf.pdf: 1501043 bytes, checksum: 4189a4e35d01ba02dd420f3bab764b2d (MD5) Previous issue date: 2009-08-28 === The biofilms microbial forms of association are responsible for generating, accelerating and / or induce the process of corrosion. The damage generated in the petroleum industry for this type of corrosion is significatives, representing major investment for your control. The aim of this study was to evaluate such tests antibiograms the effects of extracts of Jatropha curcas and essential oil of Lippia gracilis Schauer on microrganisms isolated from water samples and, thereafter, select the most effective natural product for further evaluation of biofilms formed in dynamic system. Extracts of J. curcas were not efficient on the complete inhibition of microbial growth in tests type antibiogram, and essential oil of L. gracilis Schauer most effective and determined for the other tests. A standard concentration of essential oil of 20 μL was chosen and established for the evaluation of the biofilms and the rate of corrosion. The biocide effect was determined by microbial counts of five types of microorganisms: aerobic bacteria, precipitating iron, total anaerobic, sulphate reducers (BRS) and fungi. The rate of corrosion was measured by loss of mass. Molecular identification and scanning electron microscopy (SEM) were performed. The data showed reduction to zero of the most probable number (MPN) of bacteria precipitating iron and BRS from 115 and 113 minutes of contact, respectively. There was also inhibited in fungi, reducing to zero the rate of colony-forming units (CFU) from 74 minutes of exposure. However, for aerobic and anaerobic bacteria there was no significant difference in the time of exposure to the essential oil, remaining constant. The rate of corrosion was also influenced by the presence of oil. The essential oil of L. gracilis was shown to be potentially effective === Os biofilmes s?o formas de associa??o microbiana respons?veis por gerar, acelerar e/ou induzir o processo de corros?o. Os danos gerados na ind?stria de petr?leo por esse tipo de corros?o s?o significativos, representando grandes investimentos para seu controle. O objetivo desse trabalho foi avaliar em testes tipo antibiograma os efeitos dos extratos de Jatropha curcas e do ?leo essencial de Lippia gracilis Schauer sobre microrganismos isolados de amostras de ?gua e, apartir de ent?o, selecionar o produto natural mais efetivo para posterior avalia??o sobre biofilmes formados em sistema din?mico. Os extratos de J. curcas n?o foram eficientes sobre a inibi??o completa do crescimento microbiano em testes tipo antibiogramas, sendo o ?leo essencial mais efetivo e determinado para os demais testes. Uma concentra??o padr?o do ?leo essencial de 20 μL foi escolhida e estabelecida para a avalia??o sobre os biofilmes e sobre a taxa de corros?o. O efeito biocida foi determinado pela contagem microbiana de cinco tipos de microrganismos: bact?rias aer?bicas, precipitadoras de ferro, anaer?bicas, redutoras de sulfato (BRS) e fungos. A taxa de corros?o foi calculada por perda de massa. Identifica??o molecular e micrografias eletr?nicas de varredura (MEV) foram realizadas. Os dados mostraram redu??o a zero do n?mero mais prov?vel (NMP) de bact?rias precipitadoras de ferro e BRS a partir de 115 e 113 minutos de contato, respectivamente. Tamb?m houve inibi??o sobre fungos, reduzindo para zero a taxa de unidades formadoras de col?nias (UFC) a partir de 74 minutos de exposi??o. Entretanto, para bact?rias aer?bicas e anaer?bicas n?o houve diferen?a significativa para o tempo de exposi??o com o ?leo essencial, permanecendo constante. A taxa de corros?o tamb?m foi influenciada pela presen?a do ?leo. O ?leo essencial de L. gracilis Schauer mostrou-se potencialmente efetivo