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Previous issue date: 2016-08-31 === A doen?a periodontal representa um grupo de les?es imuno-inflamat?rias que afetam tanto os tecidos de prote??o como os de sustenta??o dos dentes. Seu principal agente etiol?gico ? o biofilme bacteriano, contudo a destrui??o do tecido periodontal ? provocada principalmente pela resposta exacerbada do sistema imunol?gico do hospedeiro. Estudos recentes evidenciam o efeito anti-inflamat?rio e antioxidante dos f?rmacos antidiab?ticos orais pertencentes ? classe das sulfonilur?ias sobre algumas doen?as inflamat?rias. O objetivo desse trabalho foi avaliar a a??o da gliclazida (GLI), um antidiab?tico oral que vem apresentando efeito anti-inflamat?rio e antioxidante, sobre a doen?a periodontal em ratos que foram induzidos atrav?s de ligadura. Utilizou-se 125 ratos da ra?a wistar que foram distribu?dos aleatoriamente em 5 grupos com 25 animais cada: (NL) n?o foi submetido a indu??o da doen?a periodontal, mas recebeu solu??o salina; (L) Foi submetido a indu??o da doen?a periodontal e recebeu solu??o salina; (GLI 1mg) Foi submetido a indu??o da doen?a periodontal e recebeu GLI 1 mg/kg; (GLI 5mg) Foi submetido a indu??o da doen?a periodontal e recebeu GLI 5 mg/kg; e (GLI 10mg) Foi submetido a indu??o da doen?a periodontal e recebeu GLI 10 mg/kg. Os grupos foram tratados com solu??o salina (NL e L) ou GLI (GLI 1, GLI 5 e GLI 10mg) por gavagem durante o per?odo de 10 dias. Ap?s a eutan?sia dos animais, que ocorreu no d?cimo primeiro dia, os tecidos periodontais foram removidos e passaram pelas seguintes an?lises: morfom?trica, histopatol?gica, imuno-histoqu?mica, glutationa (GSH), PCR em tempo real (RT-PCR), malonalde?do (MDA) e mieloperoxidase (MPO). Notou-se que o tratamento com GLI 1 mg/kg foi capaz de reduzir o MDA (p <0.05), IL-1? (p <0.05), TNF-? (p <0.05) e MPO (p <0.05). Reduziu ainda a express?o de MMP-2, RANK, RANKL, catepsina K, SOD e elevou a express?o de OPG. Tendo ainda a dose de GLI 1mg/kg de forma simult?nea diminu?do a express?o de NF-?B p65, de Pl3K e tamb?m do AKT em compara??o ao grupo L (p <0.01). Esses resultados denotam que a GLI na dosagem de 1mg/kg conseguiu apresentar a??o anti-inflamat?ria na doen?a periodontal induzida, pois diminuiu os efeitos da perda ?ssea alveolar, promoveu a redu??o dos n?veis de citocinas pr?-inflamat?rias e aumentou a express?o de agentes anti-inflamat?rios. === Periodontal disease is a group of immuno inflammatory diseases that have the potential to affect both the protective fabrics as support teeth, the principal etiologic agent is bacterial biofilm, but the destruction of periodontal tissue is primarily caused by excessive response the host immune system. Recent studies show anti-inflammatory and antioxidant effect of oral antidiabetic drugs belonging to the sulfonylurea class of some inflammatory diseases. The aim of this study was to evaluate the action of gliclazide (GLI), an oral antidiabetic which has shown anti-inflammatory and antioxidant effect on periodontal disease in rats that were induced by ligation. We used 125 Wistar rats were randomly divided into 5 groups of 25 animals each: (NL) inducing periodontal disease has not been submitted but received saline solution; (L) has undergone induction of periodontal disease and received saline; (GLI 1mg) inducing periodontal disease GLU has been made and received 1 mg / kg; (GLI 5mg) was subjected to induction of periodontal disease GLU and received 5 mg / kg; and (GLI 10mg) was subjected to induction of periodontal disease GLU and received 10 mg / kg. The groups were treated with saline (NL and L) or GLI (1 GLY, GLY GLY 5 and 10mg) over a period of 10 days. After sacrificing the animals, which occurred on the eleventh day, the periodontal tissues were removed and passed the following tests: morphometric histopathologic, immunohistochemical, glutathione (GSH), real-time PCR (RT-PCR), malondialdehyde (MDA) and myeloperoxidase (MPO). It is noted that treatment with GLI 1 mg / kg was able to reduce the MDA (p <0.05) IL-1? (p <0.05), TNF-? (p <0.05) and MPO (p <0.05). also decreased MMP-2 expression, RANK, RANKL, cathepsin K, SOD and increased OPG expression. Still in the dose GLI 1mg / kg simultaneously reduced NF-?B p65 expression of AKT Pl3K and also compared to the L group (p <0.01). These results show that the GLI dosage of 1mg / kg could provide the anti-inflammatory action induced periodontal disease because the effects of decreased alveolar bone loss, caused a reduction in the levels of pro-inflammatory cytokines and increase expression of anti-agents inflammatory.
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