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Previous issue date: 2012-03-21 === Introduction: The aim of this in vitro study was to evaluate the effect of calcium
hydroxide (CH), omeprazole (O) and associations of these substances against
Enterococcus faecalis, as well as to evaluate if the acid-catalysation of the O had
any influence in the results. Methods: The methodology used was adapted from
the CLSI (Clinical Laboratory and Standards Institute) macrodilution MIC
(Minimum Inhibitory Concentration) test. Antibacterial activity was evaluated
against Enterococcus faecalis (ATCC 29212). Solutions with different
concentrations of CH, associated or not with O were prepared and placed in
contact with the inocula in the culture medium Mueller Hinton. The tubes remained
in a dry-heat oven for 24h, serial dilutions were cultivated in agar plates, and after
48h, colony-forming units (CFU) mL-1 was determined. The MIC was also
evaluated. Data were statistically analyzed using ANOVA test with Tukey posthoc,
with a level of significance of 5%. Results: The MIC for CH was 32 mg mL-1,
however reduced to 16 mg mL-1 when associated with O. The O and AO had
similar behavior. Conclusion: O potentiates the effect of CH, since the
association of these drugs reduces the MIC for E. faecalis. The acidification of O,
when associated with CH in different concentrations, did not influence its effect. === Introdu??o: O objetivo deste estudo foi avaliar, in vitro, o efeito do hidr?xido de
c?lcio (HC) e do inibidor da bomba de pr?tons Omeprazol (O), isolados e
associados contra o Enterococcus faecalis, assim como avaliar se a acidifica??o
do O influenciou nesse contexto. M?todos: A metodologia foi baseada no modelo
para Concentra??o Inibit?ria M?nima (CIM) em macrodilui??o do CLSI (Clinical
Laboratory and Standards Institute). A atividade antibacteriana foi avaliada contra
o E. faecalis (ATCC 29212). Solu??es de HC, O e associa??es dessas
subst?ncias em diferentes concentra??es, foram preparadas e colocadas em
contato com o in?culo no meio de cultura Mueller Hinton. Os tubos permaneceram
em estufa por 24h, dilui??es seriadas foram semeadas em ?gar, e ap?s 48h,
realizou-se a contagem de Unidades Formadoras de Col?nia (UFCs) / mL.
Tamb?m avaliou-se a CIM das medica??es. Os dados obtidos foram submetidos
? an?lise estat?stica, empregando-se ANOVA e post-hoc de Tukey, para α=5%.
Resultados: A CIM para o HC foi de 32mg/mL, por?m quando associado ao O e
OA reduziu para 16mg/mL. O O e o OA tiveram comportamentos semelhantes.
Conclus?o: O O potencializa o efeito do HC, uma vez que a associa??o desses
medicamentos reduz a CIM para o E. faecalis. A acidifica??o do O, quando
associado ao HC nas diferentes concentra??es, n?o influenciou o seu efeito.
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