Efeitos da exposi??o ao glifosato sobre par?metros comportamentais em peixe-zebra (Danio rerio)

• Main Author: Bridi, Daiane
• Other Authors: Bonan, Carla Denise
• Format: Others
• Language: Portuguese
• Published: Pontif?cia Universidade Cat?lica do Rio Grande do Sul 2017
• Subjects: Danio rerio; Glifosato; Peixe-zebra; Roundup?; Toxicidade; CIENCIAS DA SAUDE::FARMACIA
• Online Access: http://tede2.pucrs.br/tede2/handle/tede/7700

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No. of bitstreams: 1 DAIANE_BRIDI-DIS.pdf: 2205656 bytes, checksum: 0d6301199a5443bf6ca3d070c2d27d6e (MD5) Previous issue date: 2017-08-11 === Glyphosate has become the most widely used herbicide in the world, due to the wide scale adoption of resistant crops, after its introduction in 1996. Glyphosate can be used alone, but is commonly used as an active ingredient of the Roundup? herbicide. This herbicide contains several adjuvants in addition to glyphosate, which may promote an unknown e.g. toxicity Polioxietilenamida (POEA). Zebrafish is gaining popularity in behavioral research, because of its physiological similarity to mammals, ease of manipulation, robust performance, low cost, external fertilization, transparency of embryos larval stages and rapid development. The aim of this study was to evaluate the effects of glyphosate and Roundup? on behavioral and morphological parameters in zebrafish larvae and adult. Zebrafish larvae at 3 days post-fertilization (dpf) and adults were exposed to glyphosate (0.01, 0.065, and 0.5 mg/L) and Roundup? (0.01, 0.065, and 0.5 mg/L) for 96 hours. Immediately after the treatment, behavioral parameters such as locomotor activity and aversive behavior and morphology for the larvae and locomotion, agressive behavior and memory for the adults were analyzed. Zebrafish larvae, the results indicated that there were significant differences in the locomotor activity and aversive behavior by glyphosate and Roundup? when compared to the control. However, there was a decrease in distance traveled and the time spent in zone without stimulation for exposed larvae at doses of glyphosate and Roundup?. A significant decrease in body lenght was observed for larvae exposed to Roundup? in all concentrations tested. Our findings demonstrated that glyphosate and Roundup? exposure reduced the distance traveled, the mean speed and the line crossings in the highest concentration of glyphosate (0.5 mg / L) and 0.065 and 0.5mg/L Roundup? in animals adults. We verified that Roundup?-treated adult zebrafish showed a significant impairment in memory. Our results showed that glyphosate and Roundup? had an effect on agressive behavior. Our findings demonstrated that the effects of isolated and commercial forms of glyphosate promoted differences on locomotion, behavior and morphology of the treated animal, suggesting similar mechanisms of toxicity and cellular response. === O glifosato tornou-se o herbicida mais utilizado no mundo, devido ? ado??o ampla de culturas resistentes, ap?s sua introdu??o em 1996. O glifosato pode ser usado sozinho, mas ? comumente utilizado como ingrediente ativo do herbicida Roundup?. Este herbicida cont?m v?rios adjuvantes, tal como a Polioxietilenamida (POEA), que podem promover uma toxicidade desconhecida. O peixe-zebra est? ganhando popularidade na pesquisa comportamental, devido ? similaridade fisiol?gica com os mam?feros, facilidade de manipula??o, baixo custo, fertiliza??o externa, transpar?ncia de embri?es nos est?gios larvais e desenvolvimento r?pido. O objetivo deste estudo foi avaliar os efeitos do glifosato e do Roundup? sobre par?metros comportamentais e morfol?gicos em peixe-zebra no est?gio larval e adulto. As larvas com 3 dias p?s-fertiliza??o (dpf) e adultos foram expostos ao glifosato (0,01, 0,065 e 0,5 mg/L) e Roundup? (0,01, 0,065 e 0,5 mg/L) por 96 horas. Imediatamente ap?s o tratamento, realizamos a an?lise de par?metros comportamentais, como atividade locomotora, comportamento aversivo e morfologia para larvas e locomo??o, comportamento agressivo e mem?ria aversiva para adultos. Nas larvas houveram diferen?as significativas na atividade locomotora e comportamento aversivo nos animais tratados com glifosato e Roundup? quando comparado ao controle. Foi observada uma diminui??o na dist?ncia percorrida e na resposta aversiva nas larvas expostas ao glifosato e Roundup?. Observou-se uma diminui??o significativa no comprimento corporal das larvas expostas ao Roundup? em todas as concentra??es testadas. Nossos resultados demonstraram que a exposi??o ao glifosato ou Roundup? reduziu a dist?ncia percorrida, a velocidade m?dia e o n?mero de cruzamentos na maior concentra??o de glifosato (0,5mg/L) e 0,065 e 0,5mg/L de Roundup? em animais adultos. Verificamos que peixe-zebra adulto tratado com Roundup? apresentou um comprometimento significativo na mem?ria. Nossos resultados demostraram que o glifosato e o Roundup? tiveram efeito sobre o comportamento agressivo. Assim, nossos achados demonstraram que os efeitos das formas isoladas e comerciais de glifosato promoveram diferen?as na locomo??o, comportamento e morfologia do animal tratado, sugerindo mecanismos semelhantes de toxicidade e resposta celular.