Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury
Previous studies have reported that combination of electroacupuncture (EA) and mesenchymal stem/stromal cells (MSC) promoted survival, differentiation and functional recovery in spinal cord-transected rats. In this study, it was examined the therapeutic effects of stem cells from canine exfoliat...
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Language: | Portuguese |
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Universidade de São Paulo
2016
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Online Access: | http://www.teses.usp.br/teses/disponiveis/10/10132/tde-21032017-162328/ |
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Cell therapy
Complete spinal cord injury Intervertebral disc herniation Paraplegy Stem cells from exfoliated human dental pulp Células-tronco de polpa de dente humano esfoliado Hérnia de disco intervertebral Paraplegia Terapia Celular |
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Cell therapy
Complete spinal cord injury Intervertebral disc herniation Paraplegy Stem cells from exfoliated human dental pulp Células-tronco de polpa de dente humano esfoliado Hérnia de disco intervertebral Paraplegia Terapia Celular César Vinicius Gil Braz do Prado Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury |
description |
Previous studies have reported that combination of electroacupuncture (EA) and mesenchymal stem/stromal cells (MSC) promoted survival, differentiation and functional recovery in spinal cord-transected rats. In this study, it was examined the therapeutic effects of stem cells from canine exfoliated dental pulp (SCED) combined with EA treatment in dogs with chronic naturally occurred spinal cord injury due to intervertebral disc herniation (IVDH). Dogs were randomly assigned to four experimental groups (n=4 for each group; total of 16 animals): SCED, EA, SCED + EA) and control. Mild increase in the neurological scoring was found in one animal from SCED group (1/4; 2 points gained), one from EA group (1/4; 8 points gained), three from SCED+EA group (3/4; 16 points gained) and one from control group (1/4; 2 points gained). Functional outcome improvements were observed two animals from SCED group (2/4; 3 points gained), two from EA group (2/4; 4 points gained), one from SCED+EA group (1/4; 1 point gained) and two were from control group (2/4; 6 points gained). However no statistical differences were observed. Magnetic resonance imaging (MRI) findings did not suggest improvement comparing pre- and post-treatment within groups, excepted from one animal from SCED group (1/4), and 10 animals from all groups (10/16) presented signs of injury progression in the SCI in post-treatment exam, which could not be associated to the procedures from study, but could be related to the natural evolution of the disease. Limitation such as number of transplanted stem cells, delivery route, injury chronicity and intrinsic variation among naturally spinal cord injured dogs could have influence outcomes negatively. Moreover, canine deciduous exfoliated teeth were easily obtained and SCED were simply isolated, and no mortality followed up 7 month from procedure were observed.
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Estudos anteriores demonstraram que a associação da eletroacupuntura e células-tronco mesenquimais/estromais (CTMs) pode promover a sobrevivência e diferenciação das CTMs, assim como recuperação funcional em ratos com transecção da medula espinal. Neste estudo, foram avaliados os efeitos terapêuticos da associação de células-tronco derivadas de polpa de dente decíduo esfoliado de cães (CPDEc) e eletroacupuntura (EAP) em cães com lesão de medula espinhal crônica causada de forma natural por herniação do disco interververtebral. Os cães foram divididos aleatoriamente em quatro grupos experimentais (n=4 para cada grupo; total de 16 animais): CPDEc, EAP, CPDEc+EAP e grupo controle. Foram encontradas pequenas melhoras na pontuação do exame neurológico em um animal do grupo CPDEc (1/4; 2 pontos ganhos), um do grupo EAP (1/4; 8 pontos ganhos), três do grupo CPDEc+EAP (3/4; 16 pontos ganhos) e um do grupo controle (1/4; 2 pontos ganhos). Na avaliação funcional, pequenas melhoras também foram observadas em dois animais do grupo CPDEc (2/4; 3 pontos ganhos), dois do grupo EAP (2/3; 4 pontos ganhos), um do grupo CPDEc+EAP (1/4; 1 ponto ganho) e dois do grupo controle (2/4; 6 pontos ganhos). No entanto, não foram encontradas diferenças estatísticas entre os grupos. Os achados ressonância magnética não sugeriram melhoras comparando os exames pré e pós tratamento entre os grupos, com exceção de um animal do grupo CPDEc (1/4), e 10 animais dentre todos os grupos (10/16) apresentaram sinais de progressão na lesão da medula espinhal, que não puderam ser associados com os procedimentos do estudo, mas podem estar relacionados à progressão natural da doença. Além disso, os dentes decíduos esfoliados foram obtidos facilmente e as CPDEc foram isoladas de forma simples, ademais, não foi observada nenhuma mortalidade foi observada até 7 meses após o procedimento.
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author2 |
Maria Angélica Miglino |
author_facet |
Maria Angélica Miglino César Vinicius Gil Braz do Prado |
author |
César Vinicius Gil Braz do Prado |
author_sort |
César Vinicius Gil Braz do Prado |
title |
Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury
|
title_short |
Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury
|
title_full |
Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury
|
title_fullStr |
Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury
|
title_full_unstemmed |
Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury
|
title_sort |
combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury |
publisher |
Universidade de São Paulo |
publishDate |
2016 |
url |
http://www.teses.usp.br/teses/disponiveis/10/10132/tde-21032017-162328/ |
work_keys_str_mv |
AT cesarviniciusgilbrazdoprado combinationofstemcellsfromdeciduousteethandelectroacupunctureindogswithchronicspinalcordinjury AT cesarviniciusgilbrazdoprado associacaodecelulastroncodepolpadedentedeciduoeeletroacupunturaemcaescomlesaomedularcronica |
_version_ |
1718887968530235392 |
spelling |
ndltd-IBICT-oai-teses.usp.br-tde-21032017-1623282019-01-21T21:48:16Z Combination of stem cells from deciduous teeth and electroacupuncture in dogs with chronic spinal cord injury Associação de células-tronco de polpa de dente decíduo e eletroacupuntura em cães com lesão medular crônica César Vinicius Gil Braz do Prado Maria Angélica Miglino Alexander Birbrair Paula Fratini Peter Lees Pearson José Guilherme Xavier Cell therapy Complete spinal cord injury Intervertebral disc herniation Paraplegy Stem cells from exfoliated human dental pulp Células-tronco de polpa de dente humano esfoliado Hérnia de disco intervertebral Paraplegia Terapia Celular Previous studies have reported that combination of electroacupuncture (EA) and mesenchymal stem/stromal cells (MSC) promoted survival, differentiation and functional recovery in spinal cord-transected rats. In this study, it was examined the therapeutic effects of stem cells from canine exfoliated dental pulp (SCED) combined with EA treatment in dogs with chronic naturally occurred spinal cord injury due to intervertebral disc herniation (IVDH). Dogs were randomly assigned to four experimental groups (n=4 for each group; total of 16 animals): SCED, EA, SCED + EA) and control. Mild increase in the neurological scoring was found in one animal from SCED group (1/4; 2 points gained), one from EA group (1/4; 8 points gained), three from SCED+EA group (3/4; 16 points gained) and one from control group (1/4; 2 points gained). Functional outcome improvements were observed two animals from SCED group (2/4; 3 points gained), two from EA group (2/4; 4 points gained), one from SCED+EA group (1/4; 1 point gained) and two were from control group (2/4; 6 points gained). However no statistical differences were observed. Magnetic resonance imaging (MRI) findings did not suggest improvement comparing pre- and post-treatment within groups, excepted from one animal from SCED group (1/4), and 10 animals from all groups (10/16) presented signs of injury progression in the SCI in post-treatment exam, which could not be associated to the procedures from study, but could be related to the natural evolution of the disease. Limitation such as number of transplanted stem cells, delivery route, injury chronicity and intrinsic variation among naturally spinal cord injured dogs could have influence outcomes negatively. Moreover, canine deciduous exfoliated teeth were easily obtained and SCED were simply isolated, and no mortality followed up 7 month from procedure were observed. Estudos anteriores demonstraram que a associação da eletroacupuntura e células-tronco mesenquimais/estromais (CTMs) pode promover a sobrevivência e diferenciação das CTMs, assim como recuperação funcional em ratos com transecção da medula espinal. Neste estudo, foram avaliados os efeitos terapêuticos da associação de células-tronco derivadas de polpa de dente decíduo esfoliado de cães (CPDEc) e eletroacupuntura (EAP) em cães com lesão de medula espinhal crônica causada de forma natural por herniação do disco interververtebral. Os cães foram divididos aleatoriamente em quatro grupos experimentais (n=4 para cada grupo; total de 16 animais): CPDEc, EAP, CPDEc+EAP e grupo controle. Foram encontradas pequenas melhoras na pontuação do exame neurológico em um animal do grupo CPDEc (1/4; 2 pontos ganhos), um do grupo EAP (1/4; 8 pontos ganhos), três do grupo CPDEc+EAP (3/4; 16 pontos ganhos) e um do grupo controle (1/4; 2 pontos ganhos). Na avaliação funcional, pequenas melhoras também foram observadas em dois animais do grupo CPDEc (2/4; 3 pontos ganhos), dois do grupo EAP (2/3; 4 pontos ganhos), um do grupo CPDEc+EAP (1/4; 1 ponto ganho) e dois do grupo controle (2/4; 6 pontos ganhos). No entanto, não foram encontradas diferenças estatísticas entre os grupos. Os achados ressonância magnética não sugeriram melhoras comparando os exames pré e pós tratamento entre os grupos, com exceção de um animal do grupo CPDEc (1/4), e 10 animais dentre todos os grupos (10/16) apresentaram sinais de progressão na lesão da medula espinhal, que não puderam ser associados com os procedimentos do estudo, mas podem estar relacionados à progressão natural da doença. Além disso, os dentes decíduos esfoliados foram obtidos facilmente e as CPDEc foram isoladas de forma simples, ademais, não foi observada nenhuma mortalidade foi observada até 7 meses após o procedimento. 2016-12-20 info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/doctoralThesis http://www.teses.usp.br/teses/disponiveis/10/10132/tde-21032017-162328/ por info:eu-repo/semantics/openAccess Universidade de São Paulo Anatomia dos Animais Domésticos e Silvestres USP BR reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo instacron:USP |