Efeito de inibidores de endonucleases na transferência gênica mediada por espermatozoides em camundongos

Efeito de inibidores de endonucleases na transferência gênica mediada por espermatozoides em camundongos

A baixa eficiência e a dificuldade de reprodução de resultados da técnica de transferência gênica mediada por espermatozoides (TGME) têm como possível explicação à ativação de endonucleases espermáticas. Assim, a inibição desta enzima poderia evitar a fragmentação de DNA (exógeno e genômico), po...

Full description

Bibliographic Details
Main Author:	Fernanda Sevciuc Maria
Other Authors:	Mayra Elena Ortiz D\'Ávila Assumpção
Language:	Portuguese
Published:	Universidade de São Paulo 2012
Subjects:	ATA COMETA alcalino PCR em tempo real PCX-EGFP Transgenia animal Animal transgenesis COMET assay Real time PCR
Online Access:	http://www.teses.usp.br/teses/disponiveis/10/10131/tde-23042013-103122/

Similar Items

Efeito de inibidores de endonucleases na transferência gênica mediada por espermatozoides em camundongos
by: Maria, Fernanda Sevciuc
Published: (2012)

FID Fast Image Display for (.BMP & .PCX) Images
by: Rawaa Qasha, et al.
Published: (2004-05-01)

Selection of the Most Appropriate Medium for Assessing Motility and DNA Uptake of Bovine Spermatozoa
by: Sh. Eghbalsaied, et al.
Published: (2009-01-01)

Espermatozóide bovino como vetor de transgene: interação do DNA exógeno e viabilidade espermática
by: Weber Beringui Feitosa
Published: (2006)

Espermatozóide bovino como vetor de transgene: interação do DNA exógeno e viabilidade espermática
by: Feitosa, Weber Beringui
Published: (2006)

Visualising functionally specific neurones in living brain using c-fos expression
by: Stokes, Christabel E. L.
Published: (2003)

Simultaneous Determination and Risk Assessment of Pyrrolizidine Alkaloids in Artemisia capillaris Thunb. by UPLC-MS/MS Together with Chemometrics
by: Li-Hua Chen, et al.
Published: (2019-03-01)

ÄTA-arbeten på Trafikverket
by: Magnusson, Carl
Published: (2014)

Identification and Characterization of Three Novel Small Interference RNAs That Effectively Down-Regulate the Isolated Nucleocapsid Gene Expression of SARS Coronavirus
by: Ying-Li Cao, et al.
Published: (2011-02-01)

Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel Targeting Sites
by: Li Liu, et al.
Published: (2010-10-01)

Modificação de células-tronco espermatogoniais para produção de bovinos transgênicos
by: Flavia Regina Oliveira de Barros
Published: (2012)

Modificação de células-tronco espermatogoniais para produção de bovinos transgênicos
by: Barros, Flavia Regina Oliveira de
Published: (2012)

Hantering av ÄTA-arbeten enligt standardavtalet AB 04
by: Sager, Wisam
Published: (2014)

Computational Analysis of Chromophore Tripeptides FollowingFusion of Enhanced Green Fluorescent Protein and Cell-FollowingFusion of Enhanced Green Fluorescent Protein and Cell-penetrating Peptides penetrating Peptid
by: Silvia Tri Widyaningtyas, et al.
Published: (2020-12-01)

An improved respiratory syncytial virus neutralization assay based on the detection of green fluorescent protein expression and automated plaque counting
by: van Remmerden Yvonne, et al.
Published: (2012-10-01)

Improving the temporal resolution of a microspectrometer for the study of the photophysics of enhanced green fluorescent protein
by: Rane, Lukas
Published: (2021)

Survival in soil and detection of co-transformed Trichoderma harzianum by nested PCR Sobrevivência em solo e detecção de co-transformantes de Trichoderma harzianum por PCR "nested"
by: Paulo Roberto Queiroz, et al.
Published: (2004-04-01)

INTENSIFIKASI PEMUNGUTAN BEA PEROLEHAN HAK ATAS TANAH DAN BANGUNAN (BPHTB) DI KOTA SEMARANG BERDASARKAN PERDA KOTA SEMARANG NOMOR 2 TAHUN 2011
by: Rina Leidyawaty Silitonga
Published: (2012-06-01)

Construction and identification of EGFPSNAT2 fusion protein in mammalian expression vector
by: LI Yang, et al.
Published: (2012-10-01)

Lösningen På ÄTA-Problematiken : En kvalitativ marknadsundersökning för B2B-applikationen DEMA
by: Ring, Rebecka, et al.
Published: (2020)

Sensitive Detection of Measles Virus Infection in the Blood and Tissues of Humanized Mouse by One-step Quantitative RT-PCR
by: Shota eIkeno, et al.
Published: (2013-10-01)

The Minutes Genre and the Prepositions: Reflections Upon the Teaching of Spanish to Secretariat
by: Viviane Cristina Poletto Lugli
Published: (2017-01-01)

ÄTA-arbeten : - En otillåten direktupphandling?
by: Heinefeldt, Moa
Published: (2016)

KEDUDUKAN PERJANJIAN PENGIKATAN JUAL BELI HAK ATAS TANAH DALAM PERSPEKTIF HUKUM PERTANAHAN
by: supriyadi supriyadi
Published: (2017-08-01)

Lipsome-mediated uptake of exogenous DNA by Sahiwal cattle spermatozoa
by: Thomas V. Babu, et al.
Published: (2012-01-01)

Expressional Comparison Between Episomal and Stable Transfection of a Selected tri-fusion Protein in Leishmania tarentolae
by: Tahereh Taheri, et al.
Published: (2014-08-01)

First study of sperm mediated gene transfer in Egyptian river buffalo
by: Mohamed S. Hassanane, et al.
Published: (2017-12-01)

Toward the Development of a Single-Round Infection Assay Based on EGFP Reporting for Anti-HIV-1 Drug Discovery
by: Mahdieh Soezi, et al.
Published: (2015-10-01)

The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes
by: Dunlap-Brown, Marya
Published: (2014)

Generation and characterization of a dmdegfp reporter mouse as a tool to investigate dystrophin expression
by: Petkova, Mina
Published: (2016)

Produção de animais transgênicos por transferência nuclear como modelo de estudo biológico
by: Fabiana Fernandes Bressan
Published: (2008)

Produção de animais transgênicos por transferência nuclear como modelo de estudo biológico
by: Bressan, Fabiana Fernandes
Published: (2008)

A Khwārazmian Saint in the Golden Horde: Közlük Ata (Gözlī Ata) and the Social Vectors of Islamisation
by: Devin DeWeese
Published: (2018-10-01)

Analisa Penguasaan Hak Atas Tanah Tahun 2014 – 2015 (Studi Kasus : Kecamatan Lumajang, Kabupaten Lumajang)
by: Muhammad Irsyadi Firdaus, et al.
Published: (2017-01-01)

PENGARUH LATIHAN BEBAN BOTOL BERPASIR TERHADAP KEMAMPUAN SERVICE ATAS SISWI PESERTA EKSTRAKURIKULER BOLA VOLI DI SMPN 8 KOTA BENGKULU
by: Perdian Sutianto, et al.
Published: (2019-03-01)

Welcome to the Real World: Professional-Level Translator Certification
by: Geoffrey S Koby, et al.
Published: (2013-04-01)

Dose-dependent Toxicity of Humanized Renilla reniformis GFP (hrGFP) Limits Its Utility as a Reporter Gene in Mouse Muscle
by: Lindsay M Wallace, et al.
Published: (2013-01-01)

Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)
by: Ji Chen, et al.
Published: (2018-04-01)

PROSES PENDAFTARAN PERALIHAN HAK ATAS TANAH DALAM PERSPEKTIF KEPASTIAN HUKUM
by: Jolanda Marhel
Published: (2017-07-01)

Influência da expressão da alfa-6 integrina na produção de células-tronco espermatogoniais murinas transgênicas
by: Robinson André Worst
Published: (2012)