Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae

This work describes the phytochemical study of Hippeastrum solandriflorum(Amaryllidaceae)aiming the isolation and structural elucidation of new bioactive compounds, as well as its pharmacological investigation. The chemical investigation realized with the EtOH extract from bulbs, through chromatogra...

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Main Author: Kaline Rodrigues Carvalho
Other Authors: Maria da ConceiÃÃo de Menezes Torres
Format: Others
Language:Portuguese
Published: Universidade Federal do Cearà 2014
Subjects:
Online Access:http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13544
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spelling ndltd-IBICT-oai-www.teses.ufc.br-90272019-01-21T23:04:16Z Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae AlcalÃides bioativos e fenÃlicos de Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae Kaline Rodrigues Carvalho Maria da ConceiÃÃo de Menezes Torres Telma Leda Gomes de Lemos Kirley Marques Canuto Amaryllidaceae Hippeastrum solandriflorum AlcalÃides isoquinolÃnicos Atividade citotÃxica Amaryllidaceae Hippeastrum solandriflorum Isoquinoline alkaloids Cytotoxic activity QUIMICA DOS PRODUTOS NATURAIS This work describes the phytochemical study of Hippeastrum solandriflorum(Amaryllidaceae)aiming the isolation and structural elucidation of new bioactive compounds, as well as its pharmacological investigation. The chemical investigation realized with the EtOH extract from bulbs, through chromatographic methods, including HPLC (reverse phase), resulting inthe isolation of ten compounds: a furan derivative: 5-(hydroxymethyl)furan-2-carbaldehyde(HS-1), two phenolic derivatives: piscidic acid (HS-2), eucomic acid (HS-3), and seven isoquinoline alkaloids: narciclasin ( HS-4), 2α-hydroxypseudolycorin (HS-5), 10α-hydroxy homolycorin (HS-6), galantamin (HS-7), sanguinin (HS-8),N-oxid galantamin (HS-9) andnarcissidin (HS10). The alkaloids (HS-5) and (HS-6) are being reported for the first time inthe literature, while the other ones have been isolated for the first time in the investigated species. The structures of all isolated compounds were determined based on spectrometricmethods (IR, HRMS, NMR 1H and13Câ1D and 2D), besides comparison with published data. The cytotoxic potential of all alkaloids were evaluated against several tumor cell lines:colon (HCT-116), leukemia (HL-60), ovary (OVCAR-8) and brain (SF-295) showing IC50ranging from 0.01 to 35.7 μM. Este trabalho descreve o estudo fitoquÃmico de Hippeastrum solandriflorum (Amaryllidaceae) visando o isolamento e elucidaÃÃo estrutural de novos constituintes quÃmicos bioativos, bem como o estudo farmacolÃgico dos compostos obtidos. A investigaÃÃo quÃmica realizada com o extrato etanÃlico dos bulbos, atravÃs de mÃtodos cromatogrÃficos, incluindo CLAE (fase reversa), resultou no isolamento e identificaÃÃo de dez substÃncias, sendo um derivado do furano: 5-(hidroximetil)furan-2-carbaldeido (HS-1), dois derivados fenÃlicos: acido piscidico(HS-2), acido eucÃmico (HS-3) e sete alcaloides isoquinolÃnicos: Narciclasina (HS-4), 2α-hidroxipseudolicorina (HS-5), 10αhidroxi-homolicorina (HS-6), Galantamina (HS-7), Sanguinina (HS-8),N-oxido galantamina (HS-9), Narcissidina (HS-10). Os alcaloides (HS-5)e (HS-6) esta sendo relatado pela primeira vez na literatura e os demais como sendo inÃditos na espÃcie estudada. As substÃncias isoladas tiveram suas estruturas elucidadas por mÃtodos espectromÃtricos (IV, IES-EM e RMN de1H e13C 1D e 2D), alÃm de comparaÃÃo com dados da literatura. O potencial citotÃxicodos alcaloides isolados foi avaliado frente Ãs linhagens decÃlulas tumorais humanas: cÃlon (HCT-116), leucemia (HL-60), ovÃrio (OVCAR-8) e cÃrebro (SF-295) mostrando valores IC50 variando 0,01â35,7 μM. 2014-10-31 info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13544 por info:eu-repo/semantics/openAccess application/pdf Universidade Federal do Cearà Programa de PÃs-GraduaÃÃo em QuÃmica UFC BR reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC
collection NDLTD
language Portuguese
format Others
sources NDLTD
topic Amaryllidaceae
Hippeastrum solandriflorum
AlcalÃides isoquinolÃnicos
Atividade citotÃxica
Amaryllidaceae
Hippeastrum solandriflorum
Isoquinoline alkaloids
Cytotoxic activity
QUIMICA DOS PRODUTOS NATURAIS
spellingShingle Amaryllidaceae
Hippeastrum solandriflorum
AlcalÃides isoquinolÃnicos
Atividade citotÃxica
Amaryllidaceae
Hippeastrum solandriflorum
Isoquinoline alkaloids
Cytotoxic activity
QUIMICA DOS PRODUTOS NATURAIS
Kaline Rodrigues Carvalho
Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae
description This work describes the phytochemical study of Hippeastrum solandriflorum(Amaryllidaceae)aiming the isolation and structural elucidation of new bioactive compounds, as well as its pharmacological investigation. The chemical investigation realized with the EtOH extract from bulbs, through chromatographic methods, including HPLC (reverse phase), resulting inthe isolation of ten compounds: a furan derivative: 5-(hydroxymethyl)furan-2-carbaldehyde(HS-1), two phenolic derivatives: piscidic acid (HS-2), eucomic acid (HS-3), and seven isoquinoline alkaloids: narciclasin ( HS-4), 2α-hydroxypseudolycorin (HS-5), 10α-hydroxy homolycorin (HS-6), galantamin (HS-7), sanguinin (HS-8),N-oxid galantamin (HS-9) andnarcissidin (HS10). The alkaloids (HS-5) and (HS-6) are being reported for the first time inthe literature, while the other ones have been isolated for the first time in the investigated species. The structures of all isolated compounds were determined based on spectrometricmethods (IR, HRMS, NMR 1H and13Câ1D and 2D), besides comparison with published data. The cytotoxic potential of all alkaloids were evaluated against several tumor cell lines:colon (HCT-116), leukemia (HL-60), ovary (OVCAR-8) and brain (SF-295) showing IC50ranging from 0.01 to 35.7 μM. === Este trabalho descreve o estudo fitoquÃmico de Hippeastrum solandriflorum (Amaryllidaceae) visando o isolamento e elucidaÃÃo estrutural de novos constituintes quÃmicos bioativos, bem como o estudo farmacolÃgico dos compostos obtidos. A investigaÃÃo quÃmica realizada com o extrato etanÃlico dos bulbos, atravÃs de mÃtodos cromatogrÃficos, incluindo CLAE (fase reversa), resultou no isolamento e identificaÃÃo de dez substÃncias, sendo um derivado do furano: 5-(hidroximetil)furan-2-carbaldeido (HS-1), dois derivados fenÃlicos: acido piscidico(HS-2), acido eucÃmico (HS-3) e sete alcaloides isoquinolÃnicos: Narciclasina (HS-4), 2α-hidroxipseudolicorina (HS-5), 10αhidroxi-homolicorina (HS-6), Galantamina (HS-7), Sanguinina (HS-8),N-oxido galantamina (HS-9), Narcissidina (HS-10). Os alcaloides (HS-5)e (HS-6) esta sendo relatado pela primeira vez na literatura e os demais como sendo inÃditos na espÃcie estudada. As substÃncias isoladas tiveram suas estruturas elucidadas por mÃtodos espectromÃtricos (IV, IES-EM e RMN de1H e13C 1D e 2D), alÃm de comparaÃÃo com dados da literatura. O potencial citotÃxicodos alcaloides isolados foi avaliado frente Ãs linhagens decÃlulas tumorais humanas: cÃlon (HCT-116), leucemia (HL-60), ovÃrio (OVCAR-8) e cÃrebro (SF-295) mostrando valores IC50 variando 0,01â35,7 μM.
author2 Maria da ConceiÃÃo de Menezes Torres
author_facet Maria da ConceiÃÃo de Menezes Torres
Kaline Rodrigues Carvalho
author Kaline Rodrigues Carvalho
author_sort Kaline Rodrigues Carvalho
title Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae
title_short Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae
title_full Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae
title_fullStr Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae
title_full_unstemmed Bioactive alkaloids and phenolic Hippeastrum solandriflorum (Lindl.) - Amaryllidaceae
title_sort bioactive alkaloids and phenolic hippeastrum solandriflorum (lindl.) - amaryllidaceae
publisher Universidade Federal do CearÃ
publishDate 2014
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13544
work_keys_str_mv AT kalinerodriguescarvalho bioactivealkaloidsandphenolichippeastrumsolandriflorumlindlamaryllidaceae
AT kalinerodriguescarvalho alcalaidesbioativosefenalicosdehippeastrumsolandriflorumlindlamaryllidaceae
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