Integrated bioprocess to boost cellulosic bioethanol titers and yields

Doctor of Philosophy === Department of Biological & Agricultural Engineering === Donghai Wang === Among potential alternative liquid fuels, bioethanol is the widest utilized transportation fuels and mainly made from grains. Cellulosic biofuels provide environmental benefits not available from g...

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Main Author: Xu, Youjie
Language:en_US
Published: Kansas State University 2018
Subjects:
Online Access:http://hdl.handle.net/2097/38754
id ndltd-KSU-oai-krex.k-state.edu-2097-38754
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spelling ndltd-KSU-oai-krex.k-state.edu-2097-387542018-04-15T03:41:05Z Integrated bioprocess to boost cellulosic bioethanol titers and yields Xu, Youjie biomass biofuels pretreatment fermentation ethanol Doctor of Philosophy Department of Biological & Agricultural Engineering Donghai Wang Among potential alternative liquid fuels, bioethanol is the widest utilized transportation fuels and mainly made from grains. Cellulosic biofuels provide environmental benefits not available from grain or sugar-based biofuels and are considered as a solid foundation to meet transportation fuels needs in a low-carbon economy, albeit with electrified vehicles and other technical advances. The objective of this research was to develop and optimize various bioprocessing units to boost cellulosic bioethanol titers and yields in order to accelerate the commercialization of cellulosic bioethanol production. The results showed high-solids biomass bioconversion (12%, w/v) was inefficient in the laboratory rotary shaker. However, a horizontal reactor with good mixing was effective for high solids loading (20%, w/v), yielding 75 g/L of glucose. To achieve the minimal economical ethanol distillation requirement of 40 g/L, integrated bioprocesses were conducted to boost ethanol titers and yields through co-fermentation of starchy grain and cellulosic biomass. The maximum ethanol concentration (68.7 g/L) was achieved at the corn flour and hydrothermal-treated corn stover ratio of 12:12 using raw starch granular enzyme with the ethanol yield of 86.0%. Co-fermentation of starchy substrate with hydrolysate liquor from saccharified biomass was able to significantly enhance ethanol concentration and reduce energy cost for distillation without sacrificing ethanol yields. These results indicated integration of first and second generation ethanol production could significantly accelerate the commercialization of cellulosic biofuel production. Novel technology, modified simultaneous saccharification and fermentation, was firstly established to enhance ethanol titers and yields, which achieved high ethanol titers of 72.3 g/L at high biomass loadings of 30% (w/v) with 70.0% ethanol yield. 2018-04-09T21:25:14Z 2018-04-09T21:25:14Z 2018 May Dissertation http://hdl.handle.net/2097/38754 en_US Kansas State University
collection NDLTD
language en_US
sources NDLTD
topic biomass
biofuels
pretreatment
fermentation
ethanol
spellingShingle biomass
biofuels
pretreatment
fermentation
ethanol
Xu, Youjie
Integrated bioprocess to boost cellulosic bioethanol titers and yields
description Doctor of Philosophy === Department of Biological & Agricultural Engineering === Donghai Wang === Among potential alternative liquid fuels, bioethanol is the widest utilized transportation fuels and mainly made from grains. Cellulosic biofuels provide environmental benefits not available from grain or sugar-based biofuels and are considered as a solid foundation to meet transportation fuels needs in a low-carbon economy, albeit with electrified vehicles and other technical advances. The objective of this research was to develop and optimize various bioprocessing units to boost cellulosic bioethanol titers and yields in order to accelerate the commercialization of cellulosic bioethanol production. The results showed high-solids biomass bioconversion (12%, w/v) was inefficient in the laboratory rotary shaker. However, a horizontal reactor with good mixing was effective for high solids loading (20%, w/v), yielding 75 g/L of glucose. To achieve the minimal economical ethanol distillation requirement of 40 g/L, integrated bioprocesses were conducted to boost ethanol titers and yields through co-fermentation of starchy grain and cellulosic biomass. The maximum ethanol concentration (68.7 g/L) was achieved at the corn flour and hydrothermal-treated corn stover ratio of 12:12 using raw starch granular enzyme with the ethanol yield of 86.0%. Co-fermentation of starchy substrate with hydrolysate liquor from saccharified biomass was able to significantly enhance ethanol concentration and reduce energy cost for distillation without sacrificing ethanol yields. These results indicated integration of first and second generation ethanol production could significantly accelerate the commercialization of cellulosic biofuel production. Novel technology, modified simultaneous saccharification and fermentation, was firstly established to enhance ethanol titers and yields, which achieved high ethanol titers of 72.3 g/L at high biomass loadings of 30% (w/v) with 70.0% ethanol yield.
author Xu, Youjie
author_facet Xu, Youjie
author_sort Xu, Youjie
title Integrated bioprocess to boost cellulosic bioethanol titers and yields
title_short Integrated bioprocess to boost cellulosic bioethanol titers and yields
title_full Integrated bioprocess to boost cellulosic bioethanol titers and yields
title_fullStr Integrated bioprocess to boost cellulosic bioethanol titers and yields
title_full_unstemmed Integrated bioprocess to boost cellulosic bioethanol titers and yields
title_sort integrated bioprocess to boost cellulosic bioethanol titers and yields
publisher Kansas State University
publishDate 2018
url http://hdl.handle.net/2097/38754
work_keys_str_mv AT xuyoujie integratedbioprocesstoboostcellulosicbioethanoltitersandyields
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