A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds
Allograft tissue is used to reconstruct cardiac birth defects but induces an immune response resulting in allo-sensitization. Decellularization reduces the immune response, however, acellular vascular tissue is thrombogenic. In-vitro endothelialization may attenuate thrombogenicity. Here we offer ou...
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ndltd-LACETR-oai-collectionscanada.gc.ca-AEU.10048-5132012-07-03T12:11:11ZKorbutt, Gregory (Surgery)Desai, Leena2009-08-14T15:12:36Z2009-08-14T15:12:36Z2009-08-14T15:12:36Zhttp://hdl.handle.net/10048/513Allograft tissue is used to reconstruct cardiac birth defects but induces an immune response resulting in allo-sensitization. Decellularization reduces the immune response, however, acellular vascular tissue is thrombogenic. In-vitro endothelialization may attenuate thrombogenicity. Here we offer our work, which determines a novel method of endothelial cell attachment using Arginine-Glycine-Aspartic Acid (RGD) peptides. We show that an RGD-FITC peptide can be bound to a decellularized ovine cardiac scaffold. RGD modification increases HUVEC cell adhesion to the surface at 3 days of static incubation in-vitro compared to decellularized tissue alone. Repetition using a decellularized human scaffold shows similar results. Cleavage of the potentially immunogenic FITC label retains our RGD peptide. In summary, we determine that decellularized allografts show enhanced HUVEC cell adhesion when modified with an RGD peptide under static conditions. This may increase cell retention in-vivo leading to a decellularized cardiac allograft repopulated with functional autologous cells from the recipient.3316182 bytesapplication/pdfenTissue EngineeringDecellularizationRe-EndothelializationA Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart ScaffoldsThesisMaster of ScienceMaster'sSurgeryUniversity of Alberta2009-11Experimental SurgeryMeyer, Steven (Surgery)Ross, David (Surgery)Thebaud, Bernard (Pediatrics) |
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Tissue Engineering Decellularization Re-Endothelialization |
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Tissue Engineering Decellularization Re-Endothelialization Desai, Leena A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds |
description |
Allograft tissue is used to reconstruct cardiac birth defects but induces an immune response resulting in allo-sensitization. Decellularization reduces the immune response, however, acellular vascular tissue is thrombogenic. In-vitro endothelialization may attenuate thrombogenicity. Here we offer our work, which determines a novel method of endothelial cell attachment using Arginine-Glycine-Aspartic Acid (RGD) peptides.
We show that an RGD-FITC peptide can be bound to a decellularized ovine cardiac scaffold. RGD modification increases HUVEC cell adhesion to the surface at 3 days of static incubation in-vitro compared to decellularized tissue alone. Repetition using a decellularized human scaffold shows similar results. Cleavage of the potentially immunogenic FITC label retains our RGD peptide.
In summary, we determine that decellularized allografts show enhanced HUVEC cell adhesion when modified with an RGD peptide under static conditions. This may increase cell retention in-vivo leading to a decellularized cardiac allograft repopulated with functional autologous cells from the recipient. === Experimental Surgery |
author2 |
Korbutt, Gregory (Surgery) |
author_facet |
Korbutt, Gregory (Surgery) Desai, Leena |
author |
Desai, Leena |
author_sort |
Desai, Leena |
title |
A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds |
title_short |
A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds |
title_full |
A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds |
title_fullStr |
A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds |
title_full_unstemmed |
A Method to Enhance Re-Endothelialization of Tissue Engineered Decellularized Allograft Heart Scaffolds |
title_sort |
method to enhance re-endothelialization of tissue engineered decellularized allograft heart scaffolds |
publishDate |
2009 |
url |
http://hdl.handle.net/10048/513 |
work_keys_str_mv |
AT desaileena amethodtoenhancereendothelializationoftissueengineereddecellularizedallograftheartscaffolds AT desaileena methodtoenhancereendothelializationoftissueengineereddecellularizedallograftheartscaffolds |
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