oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse
The biological activity of α-linolenic acid (ALA) is poorly understood and primarily associated with its conversion to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). This study used the Δ6 desaturase knockout (D6KO) mouse, which lacks Δ6 desaturase and therefore cannot convert ALA, to...
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ndltd-LACETR-oai-collectionscanada.gc.ca-OGU.10214-38702013-10-04T04:13:58Zoils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouseMonteiro, Jessicapolyunsaturated fatty acidalpha-linolenic acidfatty liver diseasedelta-6 desaturaseThe biological activity of α-linolenic acid (ALA) is poorly understood and primarily associated with its conversion to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). This study used the Δ6 desaturase knockout (D6KO) mouse, which lacks Δ6 desaturase and therefore cannot convert ALA, to evaluate the independent effects of ALA on preventing non-alcoholic fatty liver disease (NAFLD). First, the capacity of very long chain fatty acids to rescue the D6KO lipid profile was established. Next, to evaluate the independent effects of ALA, D6KO or wild-type mice were fed diets containing lard, canola, flaxseed, or fish oil. Following treatment, liver phospholipid fatty acid composition was evaluated and livers were scored for steatosis and inflammation. Glucose tolerance was also evaluated. D6KO mice fed ALA-rich diets had lower liver lipid accumulation, lower hepatic inflammation (8 weeks) and improved glucose tolerance (20 weeks) relative to lard-fed D6KO mice. Overall, this thesis supports an independent biological role for ALA.D.W.L. Ma is funded by the Canola Council of Canada, Natural Sciences and Engineering Research Council of Canada and the Canada Foundation for Innovation Leaders Opportunity Fund with matching from the Ontario Research Fund; Jessica Monteiro is funded Ontario Graduate Scholarship.Ma, David2012-07-242012-08-24T18:48:40Z2012-08-24T18:48:40Z2012-08-24Thesishttp://hdl.handle.net/10214/3870en |
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en |
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polyunsaturated fatty acid alpha-linolenic acid fatty liver disease delta-6 desaturase |
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polyunsaturated fatty acid alpha-linolenic acid fatty liver disease delta-6 desaturase Monteiro, Jessica oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
description |
The biological activity of α-linolenic acid (ALA) is poorly understood and primarily associated with its conversion to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). This study used the Δ6 desaturase knockout (D6KO) mouse, which lacks Δ6 desaturase and therefore cannot convert ALA, to evaluate the independent effects of ALA on preventing non-alcoholic fatty liver disease (NAFLD). First, the capacity of very long chain fatty acids to rescue the D6KO lipid profile was established. Next, to evaluate the independent effects of ALA, D6KO or wild-type mice were fed diets containing lard, canola, flaxseed, or fish oil. Following treatment, liver phospholipid fatty acid composition was evaluated and livers were scored for steatosis and inflammation. Glucose tolerance was also evaluated. D6KO mice fed ALA-rich diets had lower liver lipid accumulation, lower hepatic inflammation (8 weeks) and improved glucose tolerance (20 weeks) relative to lard-fed D6KO mice. Overall, this thesis supports an independent biological role for ALA. === D.W.L. Ma is funded by the Canola Council of Canada, Natural Sciences and Engineering Research Council of Canada and the Canada Foundation for Innovation Leaders Opportunity Fund with matching from the Ontario Research Fund; Jessica Monteiro is funded Ontario Graduate Scholarship. |
author2 |
Ma, David |
author_facet |
Ma, David Monteiro, Jessica |
author |
Monteiro, Jessica |
author_sort |
Monteiro, Jessica |
title |
oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
title_short |
oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
title_full |
oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
title_fullStr |
oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
title_full_unstemmed |
oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
title_sort |
oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse |
publishDate |
2012 |
url |
http://hdl.handle.net/10214/3870 |
work_keys_str_mv |
AT monteirojessica oilsrichinalphalinolenicacidindependentlyprotectagainstcharacteristicsoffattyliverdiseaseinthedelta6desaturasemouse |
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1716601814652026880 |