The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus)
Considerable evidence supports anandamide (AEA) as an important mediator in the regulation of nausea and vomiting. The present study investigates the effect of inhibiting a protein reported to mediate AEA transport, FLAT (FAAH-1-like AEA transporter), on nausea and vomiting and the neural correlates...
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ndltd-LACETR-oai-collectionscanada.gc.ca-OGU.10214-72962013-10-04T04:14:29ZThe Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus)O'Brien, Lesley DEndocannabinoidAnandamideNauseaGapingVomitingFAAH-1-like anandamide transporterFLATCB1ARN272taste reactivityVisceral Insular CortexConsiderable evidence supports anandamide (AEA) as an important mediator in the regulation of nausea and vomiting. The present study investigates the effect of inhibiting a protein reported to mediate AEA transport, FLAT (FAAH-1-like AEA transporter), on nausea and vomiting and the neural correlates of AEA regulated nausea in the visceral insular cortex (VIC). The systemic administration of the AEA transport inhibitor ARN272 was evaluated in LiCl-induced conditioned gaping in rats, and vomiting in shrews. The effect of intra-cranial administration of ARN272 into the VIC was also investigated using LiCl-induced conditioned gaping in rats. Systemic administration of ARN272 dose-dependently suppressed LiCl-induced conditioned gaping in rats, and was reversed by CB1 receptor antagonism with SR141716. Systemic administration of ARN272 also attenuated vomiting in shrews. Delivery of ARN272 into the VIC produced no effect on LiCl-induced conditioned gaping in rats. These results suggest that preventing the cellular reuptake of AEA through transport inhibition tonically activates CB1 receptors to regulate toxin-induced nausea, but that this is not AEA regulated within the VIC.This research was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC-92057) to LAP.Parker, Linda2013-07-172013-08-07T13:52:37Z2013-08-07T13:52:37Z2013-08-07Thesishttp://hdl.handle.net/10214/7296en |
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en |
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topic |
Endocannabinoid Anandamide Nausea Gaping Vomiting FAAH-1-like anandamide transporter FLAT CB1 ARN272 taste reactivity Visceral Insular Cortex |
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Endocannabinoid Anandamide Nausea Gaping Vomiting FAAH-1-like anandamide transporter FLAT CB1 ARN272 taste reactivity Visceral Insular Cortex O'Brien, Lesley D The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) |
description |
Considerable evidence supports anandamide (AEA) as an important mediator in the regulation of nausea and vomiting. The present study investigates the effect of inhibiting a protein reported to mediate AEA transport, FLAT (FAAH-1-like AEA transporter), on nausea and vomiting and the neural correlates of AEA regulated nausea in the visceral insular cortex (VIC). The systemic administration of the AEA transport inhibitor ARN272 was evaluated in LiCl-induced conditioned gaping in rats, and vomiting in shrews. The effect of intra-cranial administration of ARN272 into the VIC was also investigated using LiCl-induced conditioned gaping in rats. Systemic administration of ARN272 dose-dependently suppressed LiCl-induced conditioned gaping in rats, and was reversed by CB1 receptor antagonism with SR141716. Systemic administration of ARN272 also attenuated vomiting in shrews. Delivery of ARN272 into the VIC produced no effect on LiCl-induced conditioned gaping in rats. These results suggest that preventing the cellular reuptake of AEA through transport inhibition tonically activates CB1 receptors to regulate toxin-induced nausea, but that this is not AEA regulated within the VIC. === This research was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC-92057) to LAP. |
author2 |
Parker, Linda |
author_facet |
Parker, Linda O'Brien, Lesley D |
author |
O'Brien, Lesley D |
author_sort |
O'Brien, Lesley D |
title |
The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) |
title_short |
The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) |
title_full |
The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) |
title_fullStr |
The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) |
title_full_unstemmed |
The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) |
title_sort |
impact of prolonged anandamide availability by anandamide transport inhibition on nausea-induced behaviour in rats and vomiting in shrews (suncus murinus) |
publishDate |
2013 |
url |
http://hdl.handle.net/10214/7296 |
work_keys_str_mv |
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