E. coli Fermentation for the Production of Sialic Acid

E. coli Fermentation for the Production of Sialic Acid

Show other versions (1)

Sialic acid is the terminal sugar found on most glycoproteins and is crucial in determining serum half-life and immunogenicity on glycoproteins. The scarce supply of sialic acid hinders its advancement in basic research, diagnostic development and therapeutic production. In this work, the recombinan...

Full description

Bibliographic Details
Main Author: Zhi, Li
Language:en
Published: 2013
Subjects:
sialic acid
Escherichia coli
Feeding strategies
Online Access:http://hdl.handle.net/10393/30337
id ndltd-LACETR-oai-collectionscanada.gc.ca-OOU.#10393-30337
record_format oai_dc
spelling ndltd-LACETR-oai-collectionscanada.gc.ca-OOU.#10393-303372014-06-14T03:50:34ZE. coli Fermentation for the Production of Sialic AcidZhi, Lisialic acidEscherichia coliFeeding strategiesSialic acid is the terminal sugar found on most glycoproteins and is crucial in determining serum half-life and immunogenicity on glycoproteins. The scarce supply of sialic acid hinders its advancement in basic research, diagnostic development and therapeutic production. In this work, the recombinant E. coli BRL04 (pBRL89) producing sialic acid was studied by some batch and fed batch runs of high cell density cultivation using a 3-L fermentor. Some cultivation conditions including carbon source, induction time, dissolved oxygen were optimized and different feeding strategies were compared to enhance sialic acid production. The results may be helpful to the further scale-up of sialic acid production and the production of other recombinant proteins by high cell density cultivation of E. coli.2013-12-17T21:33:00Z2013-12-17T21:33:00Z20142013-12-17Thèse / Thesishttp://hdl.handle.net/10393/30337en
collection NDLTD
language en
sources NDLTD
topic sialic acid
Escherichia coli
Feeding strategies
spellingShingle sialic acid
Escherichia coli
Feeding strategies
Zhi, Li
E. coli Fermentation for the Production of Sialic Acid
description Sialic acid is the terminal sugar found on most glycoproteins and is crucial in determining serum half-life and immunogenicity on glycoproteins. The scarce supply of sialic acid hinders its advancement in basic research, diagnostic development and therapeutic production. In this work, the recombinant E. coli BRL04 (pBRL89) producing sialic acid was studied by some batch and fed batch runs of high cell density cultivation using a 3-L fermentor. Some cultivation conditions including carbon source, induction time, dissolved oxygen were optimized and different feeding strategies were compared to enhance sialic acid production. The results may be helpful to the further scale-up of sialic acid production and the production of other recombinant proteins by high cell density cultivation of E. coli.
author Zhi, Li
author_facet Zhi, Li
author_sort Zhi, Li
title E. coli Fermentation for the Production of Sialic Acid
title_short E. coli Fermentation for the Production of Sialic Acid
title_full E. coli Fermentation for the Production of Sialic Acid
title_fullStr E. coli Fermentation for the Production of Sialic Acid
title_full_unstemmed E. coli Fermentation for the Production of Sialic Acid
title_sort e. coli fermentation for the production of sialic acid
publishDate 2013
url http://hdl.handle.net/10393/30337
work_keys_str_mv AT zhili ecolifermentationfortheproductionofsialicacid
_version_ 1716669708938248192

Similar Items