Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury
Damage to visual pathways can lead to irreversible blindness. Posterior visual pathways, located within a watershed area, are predisposed to hypoperfusion/reperfusion injury. In a novel rat model of bilateral common carotid artery occlusion (BCCAO), oxidative injury to the superior colliculus (SC)...
Main Author: | |
---|---|
Other Authors: | |
Language: | en_ca |
Published: |
2009
|
Subjects: | |
Online Access: | http://hdl.handle.net/1807/17441 |
id |
ndltd-LACETR-oai-collectionscanada.gc.ca-OTU.1807-17441 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-LACETR-oai-collectionscanada.gc.ca-OTU.1807-174412013-04-17T04:20:22ZDelayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion InjuryRamsaroop, Lynzeybilateral common carotid artery occlusionsuperior colliculusnitrotyrosinerodentretinal ganglion cellstroke0317Damage to visual pathways can lead to irreversible blindness. Posterior visual pathways, located within a watershed area, are predisposed to hypoperfusion/reperfusion injury. In a novel rat model of bilateral common carotid artery occlusion (BCCAO), oxidative injury to the superior colliculus (SC), a major visual center within the watershed area was evaluated, in addition to its effects on retinal ganglion cells (RGCs). Nitrotyrosine, a footprint of peroxynitrite-mediated oxidative injury in the SC, and microtubule-associated protein 2, a dendrite marker in the retina, were assessed using immunofluorescence and confocal microscopy. Nitrotyrosine-immunoreactivity in the SC was increased 2 weeks after BCCAO compared to controls. Microtubule-associated protein 2-immunoreactivity in the central inner plexiform layer was reduced 3 weeks after BCCAO compared to controls. Global incomplete cerebral hypoperfusion/reperfusion induced oxidative injury in the SC and retrograde RGC dendritic changes. This suggests that cerebrovascular injury affecting the posterior visual pathways may contribute to vision loss in patients.Yucel, YeniGupta, Neeru2009-032009-07-14T19:15:42ZNO_RESTRICTION2009-07-14T19:15:42Z2009-07-14T19:15:42ZThesishttp://hdl.handle.net/1807/17441en_ca |
collection |
NDLTD |
language |
en_ca |
sources |
NDLTD |
topic |
bilateral common carotid artery occlusion superior colliculus nitrotyrosine rodent retinal ganglion cell stroke 0317 |
spellingShingle |
bilateral common carotid artery occlusion superior colliculus nitrotyrosine rodent retinal ganglion cell stroke 0317 Ramsaroop, Lynzey Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury |
description |
Damage to visual pathways can lead to irreversible blindness. Posterior visual pathways, located within a watershed area, are predisposed to hypoperfusion/reperfusion injury. In a novel rat model of bilateral common carotid artery occlusion (BCCAO), oxidative injury to the superior colliculus (SC), a major visual center within the watershed area was evaluated, in addition to its effects on retinal ganglion cells (RGCs). Nitrotyrosine, a footprint of peroxynitrite-mediated oxidative injury in the SC, and microtubule-associated protein 2, a dendrite marker in the retina, were assessed using immunofluorescence and confocal microscopy. Nitrotyrosine-immunoreactivity in the SC was increased 2 weeks after BCCAO compared to controls. Microtubule-associated protein 2-immunoreactivity in the central inner plexiform layer was reduced 3 weeks after BCCAO compared to controls. Global incomplete cerebral hypoperfusion/reperfusion induced oxidative injury in the SC and retrograde RGC dendritic changes. This suggests that cerebrovascular injury affecting the posterior visual pathways may contribute to vision loss in patients. |
author2 |
Yucel, Yeni |
author_facet |
Yucel, Yeni Ramsaroop, Lynzey |
author |
Ramsaroop, Lynzey |
author_sort |
Ramsaroop, Lynzey |
title |
Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury |
title_short |
Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury |
title_full |
Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury |
title_fullStr |
Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury |
title_full_unstemmed |
Delayed Oxidative Injury to the Superior Colliculus and Retinal Changes After Cerebral Hypoperfusion/Reperfusion Injury |
title_sort |
delayed oxidative injury to the superior colliculus and retinal changes after cerebral hypoperfusion/reperfusion injury |
publishDate |
2009 |
url |
http://hdl.handle.net/1807/17441 |
work_keys_str_mv |
AT ramsarooplynzey delayedoxidativeinjurytothesuperiorcolliculusandretinalchangesaftercerebralhypoperfusionreperfusioninjury |
_version_ |
1716580726883745792 |