The role of Nck in melanoma progression /
Nck is as an adaptor protein previously implicated in actin cytoskeleton reorganization and by our laboratory, in translation and cell response to stress. Our primary objective was to determine the expression levels of Nck isotypes (Nck-1 and Nck-2) during cancer progression. We have performed weste...
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ndltd-LACETR-oai-collectionscanada.gc.ca-QMM.1018522014-02-13T04:05:53ZThe role of Nck in melanoma progression /Ismail, Salma.Melanoma -- genetics.Oncogene Proteins.Nck is as an adaptor protein previously implicated in actin cytoskeleton reorganization and by our laboratory, in translation and cell response to stress. Our primary objective was to determine the expression levels of Nck isotypes (Nck-1 and Nck-2) during cancer progression. We have performed western blot analysis of the Nck isotypes expression levels profile in various human cancer cell lines, at different stages of progression. Our data show significantly higher expression levels of Nck-2 protein in metastatic melanomas compared to non-metastatic melanomas and normal melanocytes. Using semi-quantitative RT-PCR, we demonstrated that this increase in Nck-2 expression can be also seen at the transcriptional level. The Ras/RAF/MEK/ERK pathway is often spontaneously activated in melanomas causing hyperactivation of ERK. By downregulating the expression of Nck-2 using siRNA, we have established a strong correlation between increased expression levels of Nck-2 and activated ERK. Furthermore, we have demonstrated the involvement of Nck-2 in cell proliferation and adhesion in metastatic melanomas, revealing that Nck-2 acts as a new player in this disease.McGill University2007Electronic Thesis or Dissertationapplication/pdfenalephsysno: 002653698proquestno: AAIMR38403Theses scanned by UMI/ProQuest.© Salma Ismail, 2007Master of Science (Department of Physiology.) http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101852 |
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Melanoma -- genetics. Oncogene Proteins. |
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Melanoma -- genetics. Oncogene Proteins. Ismail, Salma. The role of Nck in melanoma progression / |
description |
Nck is as an adaptor protein previously implicated in actin cytoskeleton reorganization and by our laboratory, in translation and cell response to stress. Our primary objective was to determine the expression levels of Nck isotypes (Nck-1 and Nck-2) during cancer progression. We have performed western blot analysis of the Nck isotypes expression levels profile in various human cancer cell lines, at different stages of progression. Our data show significantly higher expression levels of Nck-2 protein in metastatic melanomas compared to non-metastatic melanomas and normal melanocytes. Using semi-quantitative RT-PCR, we demonstrated that this increase in Nck-2 expression can be also seen at the transcriptional level. The Ras/RAF/MEK/ERK pathway is often spontaneously activated in melanomas causing hyperactivation of ERK. By downregulating the expression of Nck-2 using siRNA, we have established a strong correlation between increased expression levels of Nck-2 and activated ERK. Furthermore, we have demonstrated the involvement of Nck-2 in cell proliferation and adhesion in metastatic melanomas, revealing that Nck-2 acts as a new player in this disease. |
author |
Ismail, Salma. |
author_facet |
Ismail, Salma. |
author_sort |
Ismail, Salma. |
title |
The role of Nck in melanoma progression / |
title_short |
The role of Nck in melanoma progression / |
title_full |
The role of Nck in melanoma progression / |
title_fullStr |
The role of Nck in melanoma progression / |
title_full_unstemmed |
The role of Nck in melanoma progression / |
title_sort |
role of nck in melanoma progression / |
publisher |
McGill University |
publishDate |
2007 |
url |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101852 |
work_keys_str_mv |
AT ismailsalma theroleofnckinmelanomaprogression AT ismailsalma roleofnckinmelanomaprogression |
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1716644790320234496 |