Effects of Hordeum vulgare and Hordeum bulbosum genotypes, seed age, culture methods and plant growth regulators on barley haploid production

Four experiments were conducted with the objectives of (i) comparing the suitability of various H. bulbosum clones for haploid production, (ii) determining the parental effects of H. bulbosum and barley genotypes on percentage of pollinated florets yielding caryopses with rescuable embryos and on em...

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Bibliographic Details
Main Author: Qian, Jiajing
Format: Others
Language:en
Published: McGill University 1992
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Online Access:http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56678
Description
Summary:Four experiments were conducted with the objectives of (i) comparing the suitability of various H. bulbosum clones for haploid production, (ii) determining the parental effects of H. bulbosum and barley genotypes on percentage of pollinated florets yielding caryopses with rescuable embryos and on embryo viability, (iii) comparing different stages for embryo culture and caryopsis culture, and (iv) attempting to produce barley haploids directly from cultured immature caryopses. The results demonstrated: that reproductive characteristics of H. bulbosum clones varied with environmental conditions; that the hybrid H. bulbosum clones MBC-3 and MBC-4 were superior to their parents Cb2920 and Cb2929 as pollen donors; that both parental genotypes and date of harvesting after pollination had large effects on percentage of pollinated florets yielding caryopses with rescuable embryos and on embryo viability; that haploid plantlets can be generated from haploid caryopsis culture without embryo rescue, but only at a low frequency and with a slow rate of germination.