The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata»
Candida glabrata is an emerging opportunistic fungal pathogen which is the overall second leading contributor after Candida albicans in all candidiasis infections, especially in patients with compromised immune systems, such as patients who suffer from AIDS or have received organ transplants. This...
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ndltd-LACETR-oai-collectionscanada.gc.ca-QMM.870112014-02-13T03:59:14ZThe role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata»Yeung, RalphBiology - MicrobiologyCandida glabrata is an emerging opportunistic fungal pathogen which is the overall second leading contributor after Candida albicans in all candidiasis infections, especially in patients with compromised immune systems, such as patients who suffer from AIDS or have received organ transplants. This study focuses on the transcription factor activity of zinc cluster proteins, which are found only in fungi, and their relationship to antifungal resistance. In contrast to Saccharomyces cerevisiae and to some extent C. albicans, the role and mechanisms of zinc cluster proteins in drug resistance in C. glabrata has not been as thoroughly investigated in depth in the literature. The goal of this study was to generate a panel of deletion strains for putative zinc cluster genes by using a gene deletion strategy, for example in the deletion of MRR1. By using phenotypic assays on the generated knockout strains, insight regarding possible mechanistic relationships between zinc cluster proteins to drug resistance could be obtained.Candida glabrata est un mycète pathogène opportuniste et est la deuxième cause (après Candida albicans) des infections de type candidiasis, particulièrement chez les patients ayant des déficiences du système immunitaire, dont ceux souffrant du SIDA, du cancer ou ayant reçu des greffes d'organes. Cette étude se porte sur l'activité des facteurs de transcription de la famille des protéines à groupe de zinc (trouvées seulement chez les mycètes) et leur relation à la résistance antimycotique. Par contraste avec Saccharomyces cerevisiae, le rôle et les mécanismes des protéines à groupe de zinc dans la résistance aux médicaments chez C. glabrata n'ont pas été examinés en détail. Le but de cette étude était de générer des souches comportant une délétion dans chaque gène de la famille des protéines à groupe de zinc. En utilisant des essais phénotypiques sur les souches de délétions obtenues, il sera possible d'en apprendre plus sur les relations entre les protéines à groupe de zinc et la résistance aux médicaments chez C. glabrata. McGill UniversityBernard Turcotte (Internal/Supervisor)2010Electronic Thesis or Dissertationapplication/pdfenElectronically-submitted theses.All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.Master of Science (Department of Biochemistry) http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=87011 |
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Biology - Microbiology Yeung, Ralph The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata» |
description |
Candida glabrata is an emerging opportunistic fungal pathogen which is the overall second leading contributor after Candida albicans in all candidiasis infections, especially in patients with compromised immune systems, such as patients who suffer from AIDS or have received organ transplants. This study focuses on the transcription factor activity of zinc cluster proteins, which are found only in fungi, and their relationship to antifungal resistance. In contrast to Saccharomyces cerevisiae and to some extent C. albicans, the role and mechanisms of zinc cluster proteins in drug resistance in C. glabrata has not been as thoroughly investigated in depth in the literature. The goal of this study was to generate a panel of deletion strains for putative zinc cluster genes by using a gene deletion strategy, for example in the deletion of MRR1. By using phenotypic assays on the generated knockout strains, insight regarding possible mechanistic relationships between zinc cluster proteins to drug resistance could be obtained. === Candida glabrata est un mycète pathogène opportuniste et est la deuxième cause (après Candida albicans) des infections de type candidiasis, particulièrement chez les patients ayant des déficiences du système immunitaire, dont ceux souffrant du SIDA, du cancer ou ayant reçu des greffes d'organes. Cette étude se porte sur l'activité des facteurs de transcription de la famille des protéines à groupe de zinc (trouvées seulement chez les mycètes) et leur relation à la résistance antimycotique. Par contraste avec Saccharomyces cerevisiae, le rôle et les mécanismes des protéines à groupe de zinc dans la résistance aux médicaments chez C. glabrata n'ont pas été examinés en détail. Le but de cette étude était de générer des souches comportant une délétion dans chaque gène de la famille des protéines à groupe de zinc. En utilisant des essais phénotypiques sur les souches de délétions obtenues, il sera possible d'en apprendre plus sur les relations entre les protéines à groupe de zinc et la résistance aux médicaments chez C. glabrata. |
author2 |
Bernard Turcotte (Internal/Supervisor) |
author_facet |
Bernard Turcotte (Internal/Supervisor) Yeung, Ralph |
author |
Yeung, Ralph |
author_sort |
Yeung, Ralph |
title |
The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata» |
title_short |
The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata» |
title_full |
The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata» |
title_fullStr |
The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata» |
title_full_unstemmed |
The role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «Candida glabrata» |
title_sort |
role of zinc cluster protein transcription factors in regulating antifungal resistance in pathogenic yeast «candida glabrata» |
publisher |
McGill University |
publishDate |
2010 |
url |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=87011 |
work_keys_str_mv |
AT yeungralph theroleofzincclusterproteintranscriptionfactorsinregulatingantifungalresistanceinpathogenicyeastcandidaglabrata AT yeungralph roleofzincclusterproteintranscriptionfactorsinregulatingantifungalresistanceinpathogenicyeastcandidaglabrata |
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1716643007299584000 |