Identification and characterization of new cellular interacting proteins of HIV-1 integrase
HIV-1 integrase (IN) enzyme employs several viral and cellular proteins for nuclear translocation and crucial integration of viral cDNA. Successful identification of new viral/cellular interactions may shed light for better understanding of HIV-1 replication. 293T cells were transiently transfected...
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ndltd-MANITOBA-oai-mspace.lib.umanitoba.ca-1993-45252014-01-31T03:32:19Z Identification and characterization of new cellular interacting proteins of HIV-1 integrase Parvez, Md. Kamal Uddin Yao, Xiaojian (Medical Microbiology) Fowke, Keith (Medical Microbiology) Peng, Zhikang (Immunology) Cheng, keding (Human Anatomy & Cell Science) HIV-1 Integrase HIV-1 integrase (IN) enzyme employs several viral and cellular proteins for nuclear translocation and crucial integration of viral cDNA. Successful identification of new viral/cellular interactions may shed light for better understanding of HIV-1 replication. 293T cells were transiently transfected with pYEF-1-TAP-IN and cell lysate were subjected to Tandem Affinity Purification system to pull down putative IN-interacting cellular partners. A number of distinct bands from the Coomassie-stained gel were excised followed by in-gel digestion and mass spectrometry. Putative cellular partners of HIV-1 IN were heat shock protein 60 (HSP60), β-tubulin, γ-actin, ATP synthase alpha subunit and histone H1.2 were identified by mass spectrometry. Additionally, SF3A3 (splicing factor 3A3), another previously reported factor, was successfully co-immunoprecipitated with IN. The C-terminal portion of IN was found to be the region of interaction with SF3A3. Overall, this study has provided better understanding of IN dynamics enriching existing knowledge of HIV-1 IN biology. 2011-04-12T17:31:06Z 2011-04-12T17:31:06Z 2011-04-12T17:31:06Z http://hdl.handle.net/1993/4525 en_US |
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en_US |
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HIV-1 Integrase |
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HIV-1 Integrase Parvez, Md. Kamal Uddin Identification and characterization of new cellular interacting proteins of HIV-1 integrase |
description |
HIV-1 integrase (IN) enzyme employs several viral and cellular proteins for nuclear translocation and crucial integration of viral cDNA. Successful identification of new viral/cellular interactions may shed light for better understanding of HIV-1 replication. 293T cells were transiently transfected with pYEF-1-TAP-IN and cell lysate were subjected to Tandem Affinity Purification system to pull down putative IN-interacting cellular partners. A number of distinct bands from the Coomassie-stained gel were excised followed by in-gel digestion and mass spectrometry. Putative cellular partners of HIV-1 IN were heat shock protein 60 (HSP60), β-tubulin, γ-actin, ATP synthase alpha subunit and histone H1.2 were identified by mass spectrometry. Additionally, SF3A3 (splicing factor 3A3), another previously reported factor, was successfully co-immunoprecipitated with IN. The C-terminal portion of IN was found to be the region of interaction with SF3A3. Overall, this study has provided better understanding of IN dynamics enriching existing knowledge of HIV-1 IN biology. |
author2 |
Yao, Xiaojian (Medical Microbiology) |
author_facet |
Yao, Xiaojian (Medical Microbiology) Parvez, Md. Kamal Uddin |
author |
Parvez, Md. Kamal Uddin |
author_sort |
Parvez, Md. Kamal Uddin |
title |
Identification and characterization of new cellular interacting proteins of HIV-1 integrase |
title_short |
Identification and characterization of new cellular interacting proteins of HIV-1 integrase |
title_full |
Identification and characterization of new cellular interacting proteins of HIV-1 integrase |
title_fullStr |
Identification and characterization of new cellular interacting proteins of HIV-1 integrase |
title_full_unstemmed |
Identification and characterization of new cellular interacting proteins of HIV-1 integrase |
title_sort |
identification and characterization of new cellular interacting proteins of hiv-1 integrase |
publishDate |
2011 |
url |
http://hdl.handle.net/1993/4525 |
work_keys_str_mv |
AT parvezmdkamaluddin identificationandcharacterizationofnewcellularinteractingproteinsofhiv1integrase |
_version_ |
1716628969757868032 |