Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)

Fusarium head blight (FHB), a fungal disease caused principally by Fusarium graminearum, and barley yellow dwarf (BYD) caused by BYD luteoviruses are two serious fungal and viral diseases of wheat resulting in high economic losses annually. Wuhan, a Chinese wheat cultivar resistant to FHB, and Ma...

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Main Author: Pradhan, Manika Pakhrin
Other Authors: Gilbert,Jeannie (Biological Sciences)
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/1993/4806
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spelling ndltd-MANITOBA-oai-mspace.lib.umanitoba.ca-1993-48062014-01-31T03:32:35Z Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.) Pradhan, Manika Pakhrin Gilbert,Jeannie (Biological Sciences) Brûlé-Babel,Anita (Plant Science); Hausner,Georg (Microbiology); Haber,Steve(Biological Sciences); Seifers,Dallas(Kansas State University). wheat fusarium head blight Fusarium graminearum barley yellow dwarf enduring resistance Wuhan Maringa doubled haploid Fusarium head blight (FHB), a fungal disease caused principally by Fusarium graminearum, and barley yellow dwarf (BYD) caused by BYD luteoviruses are two serious fungal and viral diseases of wheat resulting in high economic losses annually. Wuhan, a Chinese wheat cultivar resistant to FHB, and Maringa, a Brazilian cultivar tolerant to BYDV were inter-crossed and crossed with Roblin, a Canada western red spring wheat susceptible to both FHB and BYDV, to determine the genetic basis of resistance/tolerance and to combine the two traits. Four hundred ninety nine F1-derived doubled haploid (DH) lines were generated from reciprocal crosses using corn pollen-mediated DH technology. The DH lines and the parents were evaluated for disease symptoms, reduction in height and spike mass for BYD and for disease incidence, disease severity and Fusarium-damaged kernels for FHB in field and controlled environments. A subset (20/150) of the best performing DH lines from Wuhan/Maringa populations for both BYD and FHB were further evaluated. Plants were point inoculated with F. graminearum in greenhouse experiments, and macroconidial spray inoculations and spread of corn inoculum were used in field environments to evaluate FHB. BYDV inoculations were performed by placing ten to fifteen viruliferous aphids (Rhopalosiphum padi infected with BYDV-PAV isolate 9301PAV), at the one to two leaf stage for both greenhouse and field trials. The studies showed that both FHB and BYDV are quantitatively inherited. Transgressive segregants were observed and the broad sense heritability was high (0.90 to 0.97) for all traits evaluated. Results from independent testing of diseases on Wuhan/ Maringa populations showed fourteen DH lines were as, or more resistant than Wuhan for FHB and Maringa for BYDV tolerance and have combined both BYDV tolerance and FHB resistance. Identifying such lines facilitates the pyramiding of independent genes to obtain adequate levels of enduring resistance. A further experiment was conducted on the 14 lines by inoculating them with BYDV and F. graminearum successively on the same plant. Six out of 14 selected DH lines demonstrated high resistance to FHB and tolerance to BYDV. These six lines can be used in FHB/BYDV resistance/tolerance breeding programs. 2011-08-31T17:17:12Z 2011-08-31T17:17:12Z 2011-08-31 http://hdl.handle.net/1993/4806
collection NDLTD
sources NDLTD
topic wheat
fusarium head blight
Fusarium graminearum
barley yellow dwarf
enduring resistance
Wuhan
Maringa
doubled haploid
spellingShingle wheat
fusarium head blight
Fusarium graminearum
barley yellow dwarf
enduring resistance
Wuhan
Maringa
doubled haploid
Pradhan, Manika Pakhrin
Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)
description Fusarium head blight (FHB), a fungal disease caused principally by Fusarium graminearum, and barley yellow dwarf (BYD) caused by BYD luteoviruses are two serious fungal and viral diseases of wheat resulting in high economic losses annually. Wuhan, a Chinese wheat cultivar resistant to FHB, and Maringa, a Brazilian cultivar tolerant to BYDV were inter-crossed and crossed with Roblin, a Canada western red spring wheat susceptible to both FHB and BYDV, to determine the genetic basis of resistance/tolerance and to combine the two traits. Four hundred ninety nine F1-derived doubled haploid (DH) lines were generated from reciprocal crosses using corn pollen-mediated DH technology. The DH lines and the parents were evaluated for disease symptoms, reduction in height and spike mass for BYD and for disease incidence, disease severity and Fusarium-damaged kernels for FHB in field and controlled environments. A subset (20/150) of the best performing DH lines from Wuhan/Maringa populations for both BYD and FHB were further evaluated. Plants were point inoculated with F. graminearum in greenhouse experiments, and macroconidial spray inoculations and spread of corn inoculum were used in field environments to evaluate FHB. BYDV inoculations were performed by placing ten to fifteen viruliferous aphids (Rhopalosiphum padi infected with BYDV-PAV isolate 9301PAV), at the one to two leaf stage for both greenhouse and field trials. The studies showed that both FHB and BYDV are quantitatively inherited. Transgressive segregants were observed and the broad sense heritability was high (0.90 to 0.97) for all traits evaluated. Results from independent testing of diseases on Wuhan/ Maringa populations showed fourteen DH lines were as, or more resistant than Wuhan for FHB and Maringa for BYDV tolerance and have combined both BYDV tolerance and FHB resistance. Identifying such lines facilitates the pyramiding of independent genes to obtain adequate levels of enduring resistance. A further experiment was conducted on the 14 lines by inoculating them with BYDV and F. graminearum successively on the same plant. Six out of 14 selected DH lines demonstrated high resistance to FHB and tolerance to BYDV. These six lines can be used in FHB/BYDV resistance/tolerance breeding programs.
author2 Gilbert,Jeannie (Biological Sciences)
author_facet Gilbert,Jeannie (Biological Sciences)
Pradhan, Manika Pakhrin
author Pradhan, Manika Pakhrin
author_sort Pradhan, Manika Pakhrin
title Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)
title_short Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)
title_full Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)
title_fullStr Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)
title_full_unstemmed Combining Fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (Triticum aestivum L.)
title_sort combining fusarium head blight resistance and barley yellow dwarf virus tolerance in spring wheat (triticum aestivum l.)
publishDate 2011
url http://hdl.handle.net/1993/4806
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