An examination of peptide-tether binding to gold and glass surfaces

• Main Author: Kunkel, Jacquelyn Anne
• Other Authors: Matthew J. Lang.
• Format: Others
• Language: English
• Published: Massachusetts Institute of Technology 2010
• Subjects: Mechanical Engineering.
• Online Access: http://hdl.handle.net/1721.1/54507

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2009. === Cataloged from PDF version of thesis. === Includes bibliographical references (p. 14). === For many biological applications, it is beneficial to know that a peptide will bind to a surface. In this thesis, a tether, bead, peptide complex is constructed using the gold binding peptide (GBP) of sequence (CGGVSGSSPDS). Several assays and assay modifications are developed and tested to attempt to attach the GBP first to a gold slide and then to gold nanoparticles. Four peptides: FO₂, K1, K2, K3, known to bind to sapphire were attached to glass to see if it was possible to modify the sapphire assay to work with glass. === by Jacquelyn Anne Kunkel. === S.B.