MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS

MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS

Bibliographic Details
Main Author: Looft, Torey P.
Language:English
Published: Bowling Green State University / OhioLINK 2005
Subjects:
Microorganisms
organic
decomposition
ecosystems
Lake Erie
dissolved organic matter
16S ribosomal DNA
phylogenetic
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1131392301
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu11313923012021-08-03T05:27:44Z MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS Looft, Torey P. Microorganisms organic decomposition ecosystems Lake Erie dissolved organic matter 16S ribosomal DNA phylogenetic Microorganisms Microorganisms perform important roles in elemental cycling and organic decomposition, which are vital for ecosystems to function. Lake Erie offers a unique opportunity to study microbial communities across a large environmental gradient. Lake Erie consist of three basins and is affected by allochthonous inputs of dissolved organic matter (DOM) that increase to the west. In addition, the Central Basin of Lake Erie is characterized by an area dominated by a Dead Zone, which experiences periodic hypoxic events. To evaluate patterns of microbial diversity, environmental samples from eleven sites were selected for PCR amplification, cloning and sequencing of 16s ribosomal DNA genes from microbial species. Samples included inshore sites from the Western, Central and Eastern Basins as well as from the Dead Zone. Characterization of environmental DNA sequences was performed through sequence database searches and phylogenetic analyses. Biodiversity indices and measures of sequence diversity were obtained. Results from this study demonstrated considerable levels of spatial variability of microbial communities throughout Lake Erie. Characterized species included bacterial groups with diverse metabolic capabilities and key members involved in the cycling of nutrients. The relative preponderance of Gammaproteobacteria in the Western and Central Basins, but not in the Eastern Basin, may reflect the presumably widespread carbon substrate range found in the Western and Central Basins due to the greater number of allochthonous inputs. East and Central Basins showed similarities in species diversity, while the Dead Zone represented the most distinct group. These results are consistent with the idea that microbial community structure may be modulated by DOM inputs and environmental conditions. 2005-11-09 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1131392301 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1131392301 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Microorganisms
organic
decomposition
ecosystems
Lake Erie
dissolved organic matter
16S ribosomal DNA
phylogenetic
Microorganisms
spellingShingle Microorganisms
organic
decomposition
ecosystems
Lake Erie
dissolved organic matter
16S ribosomal DNA
phylogenetic
Microorganisms
Looft, Torey P.
MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS
author Looft, Torey P.
author_facet Looft, Torey P.
author_sort Looft, Torey P.
title MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS
title_short MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS
title_full MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS
title_fullStr MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS
title_full_unstemmed MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN LAKE ERIE SEDIMENTS
title_sort molecular characterization of microbial communities in lake erie sediments
publisher Bowling Green State University / OhioLINK
publishDate 2005
url http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1131392301
work_keys_str_mv AT loofttoreyp molecularcharacterizationofmicrobialcommunitiesinlakeeriesediments
_version_ 1719420411676983296

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