The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland

Bibliographic Details
Main Author:	Pillai, Mahesh R.
Language:	English
Published:	Bowling Green State University / OhioLINK 2008
Subjects:	Molecular Biology PCB PCB 47/77 catecholamines dopamine epinephrine norepinephrine tyrosine hydroxylase (TH) dopamine &946 -monooxygenase (DBM) development dose response
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu12142356902021-08-03T05:28:38Z The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland Pillai, Mahesh R. Molecular Biology PCB PCB 47/77 catecholamines dopamine epinephrine norepinephrine tyrosine hydroxylase (TH) dopamine &946 -monooxygenase (DBM) development dose response Polychlorinated biphenyls (PCBs) are environmental pollutants that bioaccumulate through the food web. Lipophilic endocrine disruptors, PCBs accumulate in adipocytes, liver, muscle and breast tissues. The adrenal medulla synthesizes the catecholamines, dopamine, norepinephrine and epinephrine, using several enzymes, including tyrosine hydroxylase (TH) and dopamine β-monooxygenase (DBM). Since catecholamines are important neurotransmitters, this study investigates the effect of PCB 47/77 on DBM and TH expression in adrenal glands of 3, 21 and 60 day old rats. PCB 47/77 was administered orally from conception until the rats were euthanized in doses of 12.5 and 25 ppm. Western blot analyses showed decreased DBM expression in 3 and 60 day old rats treated with both PCB doses, while decreased DBM expression only was observed in 21 day old rats after 25 ppm PCB treatment. TH expression was lower in 3 day old rats treated with 12.5 and 25 ppm PCB as compared to the controls. However, TH expression was elevated in 21 day old rats treated with 12.5 ppm PCB, while in 60 day old rats TH expression was elevated only following 25 ppm PCB treatment. Studies to determine the effect of PCB 47/77 on the developmental expression of DBM and TH showed that in control rats, the greatest DBM and TH levels were observed in 3 day old rats followed by a decrease in expression levels with the increasing age of the rats. Following treatment with 12.5 ppm PCB, DBM expression decreased only in 60 day old rats, while a decrease in 21 and 60 day old rats was observed with 25 ppm PCB treatment as compared to 3 day old rats. In contrast, TH levels were the lowest in 3 day old rats, while a steady increase in TH levels was observed with increasing age of the animals following 25 ppm PCB treatment. Overall, exposure of rat adrenal glands to PCB 47/77 results in alterations in DBM and TH levels that could adversely affect the catecholamine synthesis needed to regulate stress-induced homeostatic mechanisms involved in the flight or fight response. 2008-08-07 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Molecular Biology PCB PCB 47/77 catecholamines dopamine epinephrine norepinephrine tyrosine hydroxylase (TH) dopamine &946 -monooxygenase (DBM) development dose response
spellingShingle	Molecular Biology PCB PCB 47/77 catecholamines dopamine epinephrine norepinephrine tyrosine hydroxylase (TH) dopamine &946 -monooxygenase (DBM) development dose response Pillai, Mahesh R. The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland
author	Pillai, Mahesh R.
author_facet	Pillai, Mahesh R.
author_sort	Pillai, Mahesh R.
title	The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland
title_short	The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland
title_full	The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland
title_fullStr	The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland
title_full_unstemmed	The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland
title_sort	effect of a 2,2',4,4'-tetrachlorobiphenyl (pcb 47) and 3,3',4,4'-tetrachlorobiphenyl (pcb 77) mixture on enzymes involved in the synthesis of catecholamines in the rat adrenal gland
publisher	Bowling Green State University / OhioLINK
publishDate	2008
url	http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690
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_version_	1719420628544520192

The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland

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