Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer
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Case Western Reserve University School of Graduate Studies / OhioLINK
2011
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1295625781 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-case12956257812021-08-03T05:33:51Z Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer Avva, Jayant Cellular Biology Engineering Systems Science systems biology time profile cell cycle control system model computational biology We present here a complex systems biology approach towards elucidating the role of mammalian cell cycle signaling in cancer. In this context, availability of copious amounts of biological data has done little to alter the paucity of contextually consistent dynamic time profile data, necessary for the calibration and validation of dynamic models of the biological systems. Such computational models form the heart of the complex systems biology approach, and paucity of appropriate data is an immediate impediment. To address this problem, we developed a novel methodology to filter measurement noise and extract time profile variation of cell cycle biochemicals from statically sampled flow cytometry data. Taking a hierarchical viewpoint, a mathematical model of the upstream signaling from the receptor through to the nucleus was developed. A computational model of the downstream cell cycle control system based on a modified Tyson’s mathematical model that uses our time profile extraction methodology for calibration was also developed. The approach was demonstrated separately using K562 and MOLT4 cell line experimental data from the wetlab. We built custom software, CytoSys, to facilitate the application of our methodology. 2011-03-17 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1295625781 http://rave.ohiolink.edu/etdc/view?acc_num=case1295625781 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Cellular Biology Engineering Systems Science systems biology time profile cell cycle control system model computational biology |
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Cellular Biology Engineering Systems Science systems biology time profile cell cycle control system model computational biology Avva, Jayant Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer |
| author |
Avva, Jayant |
| author_facet |
Avva, Jayant |
| author_sort |
Avva, Jayant |
| title |
Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer |
| title_short |
Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer |
| title_full |
Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer |
| title_fullStr |
Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer |
| title_full_unstemmed |
Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer |
| title_sort |
complex systems biology of mammalian cell cycle signaling in cancer |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2011 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1295625781 |
| work_keys_str_mv |
AT avvajayant complexsystemsbiologyofmammaliancellcyclesignalingincancer |
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1719421807790915584 |