Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding

Bibliographic Details
Main Author: Bergquist, Gregory M.
Language:English
Published: Case Western Reserve University School of Graduate Studies / OhioLINK 2012
Subjects:
VHS
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=case1338475036
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-case13384750362021-08-03T05:34:26Z Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding Bergquist, Gregory M. Animal Diseases Viral Hemorrhagic Septicemia Virus VHS VHSV Yellow Perch Lake Erie Great Lakes Following the recent detection of VHSv in the Great Lakes I conducted a study on VHSv in Lake Erie yellow perch. A newly developed RT-PCR and nested-PCR assay was tested on yellow perch blood samples collected in 2008 and compared to the cell culture method of testing. I found that the RT-PCR assay coupled with the nested PCR was more sensitive in detecting VHSv in yellow perch than the cell culture method used by testing laboratories. The effects of VHSv infection on yellow perch feeding were investigated by analysis of growth patterns in yellow perch otoliths. Periods of slow growth in the early portion of the growing season were significantly more frequent in peak-VHSv years than pre-VHSv years. Lastly, phylogenetic analysis of nucleocapsid gene sequences from VHSv showed sequence differences from Great Lakes isolates were associated with geographic location and not host species or time of isolation. 2012-08-24 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1338475036 http://rave.ohiolink.edu/etdc/view?acc_num=case1338475036 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Animal Diseases
Viral Hemorrhagic Septicemia Virus
VHS
VHSV
Yellow Perch
Lake Erie
Great Lakes
spellingShingle Animal Diseases
Viral Hemorrhagic Septicemia Virus
VHS
VHSV
Yellow Perch
Lake Erie
Great Lakes
Bergquist, Gregory M.
Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding
author Bergquist, Gregory M.
author_facet Bergquist, Gregory M.
author_sort Bergquist, Gregory M.
title Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding
title_short Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding
title_full Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding
title_fullStr Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding
title_full_unstemmed Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding
title_sort viral hemorrhagic septicemia virus (vhsv) infection in lake erie yellow perch, perca flavescens, and its effect on feeding
publisher Case Western Reserve University School of Graduate Studies / OhioLINK
publishDate 2012
url http://rave.ohiolink.edu/etdc/view?acc_num=case1338475036
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