Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes

Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes

Bibliographic Details
Main Author: Krishnan, Sushma
Language:English
Published: Case Western Reserve University School of Graduate Studies / OhioLINK 2015
Subjects:
Parasitology
plasmodium vivax
malaria
duffy antigen
duffy binding protein
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=case1427464747
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-case14274647472021-08-03T06:29:33Z Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes Krishnan, Sushma Parasitology plasmodium vivax malaria duffy antigen duffy binding protein Erythrocyte invasion by P. vivax has been considered to be dependent on the interaction between P. vivax Duffy Binding protein (PvDBP) and Duffy antigen (Fy). There exist two important single point mutations on the FY gene, which results in different Duffy genotypes and consequently varied susceptibility to vivax malaria. Our study has a two-pronged objective. Firstly, using an erythrocyte-binding assay, we show that rPvDBPII binds to the red cells in a concentration dependent manner. We observe, variation in the percent binding between donor erythrocytes of the same Duffy genotype despite similar Fy6 antigen expression. This could suggest the presence of other red blood cell polymorphisms surrounding the Fy antigen or post-translational modifications. Secondly, we see that, rPvDBPII binds to a chymotrypsin sensitive unknown receptor on Duffy negative erythrocytes. This result could imply the presence of an alternative pathway or other essential Erythrocyte Binding Proteins than PvDBP. 2015-06-03 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1427464747 http://rave.ohiolink.edu/etdc/view?acc_num=case1427464747 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.
collection NDLTD
language English
sources NDLTD
topic Parasitology
plasmodium vivax
malaria
duffy antigen
duffy binding protein
spellingShingle Parasitology
plasmodium vivax
malaria
duffy antigen
duffy binding protein
Krishnan, Sushma
Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes
author Krishnan, Sushma
author_facet Krishnan, Sushma
author_sort Krishnan, Sushma
title Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes
title_short Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes
title_full Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes
title_fullStr Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes
title_full_unstemmed Investigating the interaction between rPvDBPII and duffy antigen on human erythrocytes
title_sort investigating the interaction between rpvdbpii and duffy antigen on human erythrocytes
publisher Case Western Reserve University School of Graduate Studies / OhioLINK
publishDate 2015
url http://rave.ohiolink.edu/etdc/view?acc_num=case1427464747
work_keys_str_mv AT krishnansushma investigatingtheinteractionbetweenrpvdbpiiandduffyantigenonhumanerythrocytes
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