Development of nanoparticle probes for magnetic particle spectrometry and thermal applications.
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Case Western Reserve University School of Graduate Studies / OhioLINK
2021
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ndltd-OhioLink-oai-etd.ohiolink.edu-case16197089804262292021-08-03T07:17:18Z Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. Ju, Minseon Chemistry superparamagnetic nanoparticles magnetic particle spectrometry magnetic resonance imaging magnetic hyperthermia bacterial biofilm Iron oxide nanoparticles (IONPs) are widely investigated due to their unique, tunable magnetic properties, and promising applications in environmental monitoring and biomedical applications. However, there are current limitations in the use of IONPs in real world applications including the lack of reproducibility in the synthetic approach to prepare biocompatible IONPs for in vivo applications. In this thesis, I have focused on engineering the size, shape, chemical composition, and surface chemistry of IONPs to optimize them as tracers for magnetic particle spectrometry and magnetic hyperthermia applications. In Chapters 2 and 3, I present my research on demonstrating the effect of particle size, shape, and surface chemistry on the magnetic particle imaging (MPI) performance of IONPs. On the other hand, in Chapters 4 and 5, my research work centers on optimizing IONPs for magnetic thermal heating applications.Chapter 2 presents my research work on modifying the surface chemistry of different sized IONPs with ethylenediaminetetraacetic acid (EDTA) to monitor their uptake and translocation in garden cress plants, using the magnetic particle spectrometry (MPS) approach. The plants treated with IONP-EDTA showed larger leaves (8-fold increase in biomass) in comparison to the control plants and the plants treated with the Fe-EDTA fertilizer. In Chapter 3, the magnetic response of a series of spatially arranged cubic-shaped IONP clusters (i.e. chain-like dimer/trimer, centrosymmetric clusters, and enzymatically cleavable two-dimensional clusters) was investigated with magnetic particle and magnetic resonance relaxometry. The chain-like dimer/trimer clusters showed 36% enhancement in MPI response compared to the commercial MPI tracer, VivotraxTM. This chapter also demonstrates the time-dependent magnetic response of magnetic clusters upon exposure to a lytic enzyme, which can further be used to trace the disassembly of the large planar clusters into smaller nanocube chains by enzymatic polymer degradation. In Chapter 4, a two-step approach using magnetic nanocomposite loaded with D-amino acid and IONPs in a thermoresponsive glycol chitin-based hydrogel (MagDAA gel), was developed to completely disrupt pre-formed bacterial biofilms of Staphylococcus aureus (S. aureus). The shape anisotropy of IONPs is exploited to maximize the heating performance of the IONPs under AC magnetic field excitation. To further investigate the role of our developed thermoresponsive hydrogel, in Chapter 5, my research involved the development of a rabbit animal model to represent prosthetic joint infections (PJIs), which was done in collaboration with the Cleveland Clinic. In summary, this thesis provides a systematic investigation on the effects of particle size, shape, and surface chemistry of IONPs for their development as nanoprobes for environmental and biomedical, as well as their use as magnetically-actuated thermal agents for antibacterial applications. 2021-06-21 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1619708980426229 http://rave.ohiolink.edu/etdc/view?acc_num=case1619708980426229 restricted--full text unavailable until 2023-05-30 This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Chemistry superparamagnetic nanoparticles magnetic particle spectrometry magnetic resonance imaging magnetic hyperthermia bacterial biofilm |
| spellingShingle |
Chemistry superparamagnetic nanoparticles magnetic particle spectrometry magnetic resonance imaging magnetic hyperthermia bacterial biofilm Ju, Minseon Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| author |
Ju, Minseon |
| author_facet |
Ju, Minseon |
| author_sort |
Ju, Minseon |
| title |
Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| title_short |
Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| title_full |
Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| title_fullStr |
Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| title_full_unstemmed |
Development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| title_sort |
development of nanoparticle probes for magnetic particle spectrometry and thermal applications. |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2021 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1619708980426229 |
| work_keys_str_mv |
AT juminseon developmentofnanoparticleprobesformagneticparticlespectrometryandthermalapplications |
| _version_ |
1719458281943990272 |