Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory
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Kent State University / OhioLINK
2011
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-kent13225012142021-08-03T05:37:44Z Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory Bhatta, Fanindra P. Biophysics Physics Solid State Physics Phase separation mixed lipid monolayers and bilayers Brewster angle microscopy fluorescence microscopy Landau free energy This dissertation looks at the phase-separation of two binary lipid systems: (a) an experimental study of binary lipid Langmuir monolayers and (b) the theoretical modeling of a binary system of lipids in a symmetric bilayer.Experimentally, monolayers of dihydrocholesterol (Dchol) and dimyristoylphosphatidylcholine (DMPC) at the air/water interface were studied with Brewster angle microscopy (BAM) and fluorescence microscopy (FM) in order to test the effect of fluorescent dyes, which may be strongly line active, on the phase diagram. The theoretical model of phase separation in mixed lipid bilayers emphasizes the asymmetry of the two layers of a bilayer. A Landau free energy in terms of order parameters the cholesterol mole fraction, and the hydrocarbon chain length is extended to look at both cubic and quartic terms. We found four states: (1) a single homogeneous phase, (2) two-phase coexistence, (3) three-phase coexistence, and (4) four-phase coexistence, depending on temperature and the composition of the layers. Three phase and four phase coexistence are of special interest because they have been little studied either theoretically or experimentally. 2011-11-28 English text Kent State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214 http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biophysics Physics Solid State Physics Phase separation mixed lipid monolayers and bilayers Brewster angle microscopy fluorescence microscopy Landau free energy |
| spellingShingle |
Biophysics Physics Solid State Physics Phase separation mixed lipid monolayers and bilayers Brewster angle microscopy fluorescence microscopy Landau free energy Bhatta, Fanindra P. Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory |
| author |
Bhatta, Fanindra P. |
| author_facet |
Bhatta, Fanindra P. |
| author_sort |
Bhatta, Fanindra P. |
| title |
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory |
| title_short |
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory |
| title_full |
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory |
| title_fullStr |
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory |
| title_full_unstemmed |
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory |
| title_sort |
phase separation in binary lipid monolayers bilayers: experiment and theory |
| publisher |
Kent State University / OhioLINK |
| publishDate |
2011 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214 |
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AT bhattafanindrap phaseseparationinbinarylipidmonolayersbilayersexperimentandtheory |
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