GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells

GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells

Bibliographic Details
Main Author: Kesiry, Riad
Language:English
Published: University of Toledo Health Science Campus / OhioLINK 2004
Subjects:
GRP78/BiP
ouabain
proteins
Na/K-ATPase
LLC-PK1
LLC-PK1 Cells
mmol/L
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=mco1096302498
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-mco10963024982021-08-03T05:38:36Z GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells Kesiry, Riad GRP78/BiP ouabain proteins Na/K-ATPase LLC-PK1 LLC-PK1 Cells mmol/L We have demonstrated that ouabain causes dose- and time-dependent decreases both in 86Rb uptake and plasmalemmal Na/K-ATPase content of LLC-PK1 cells (Liu et al., 2002). This phenomenon is related to ouabain-induced endocytosis of plasmalemmal Na/K-ATPase in LLC-PK1 Cells by a clathrin-dependent mechanism (Liu et al., 2004). GRP78/BiP is a resident protein of the endoplasmic reticulum (ER) and acts as a molecular chaperone. Recently, several studies have shown GRP78/BiP is also expressed on the cell surface and forms heterogeneous, high molecular weight complexes with other proteins. To identify the proteins that are possibly involved in ouabain-induced endocytosis of the Na/K-ATPase in LLC-PK1 cells, we separated and identified endosomal proteins by 2D gel electrophoresis and MS/MS from both control and ouabain-treated LLC-PK1 cells. GRP78/BiP was identified by MS/MS as one of several up-regulated proteins and confirmed by Western Blot. Interestingly, GRP78/BiP was significantly up-regulated in both early and late endosomes in response to ouabain treatment. By using a cell surface protein biotinylation technique to isolate the cell surface membrane proteins, we found that GRP/BiP is expressed on the cell surface of LLC-PK1 cells and down-regulated in a time-dependent manner in response to ouabain. By comparing the cellular redistribution, our data suggest that both the Na/K-ATPase a-1 subunit and GRP78/BiP follow the same pattern in response to ouabain. 2004-09-27 English text University of Toledo Health Science Campus / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=mco1096302498 http://rave.ohiolink.edu/etdc/view?acc_num=mco1096302498 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic GRP78/BiP
ouabain
proteins
Na/K-ATPase
LLC-PK1
LLC-PK1 Cells
mmol/L
spellingShingle GRP78/BiP
ouabain
proteins
Na/K-ATPase
LLC-PK1
LLC-PK1 Cells
mmol/L
Kesiry, Riad
GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells
author Kesiry, Riad
author_facet Kesiry, Riad
author_sort Kesiry, Riad
title GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells
title_short GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells
title_full GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells
title_fullStr GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells
title_full_unstemmed GRP78/BiP is Involved in Ouabain-induced Endocytosis of the Na/K-ATPase in LLC-PK1 Cells
title_sort grp78/bip is involved in ouabain-induced endocytosis of the na/k-atpase in llc-pk1 cells
publisher University of Toledo Health Science Campus / OhioLINK
publishDate 2004
url http://rave.ohiolink.edu/etdc/view?acc_num=mco1096302498
work_keys_str_mv AT kesiryriad grp78bipisinvolvedinouabaininducedendocytosisofthenakatpaseinllcpk1cells
_version_ 1719422921460416512

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