Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases

Bibliographic Details
Main Author:	Alazizi, Adnan
Language:	English
Published:	University of Toledo Health Science Campus / OhioLINK 2011
Subjects:	Animal Sciences Biochemistry Biomedical Research Pharmacology Toxicology Zebrafish Catechol O-methyltransferases COMT Methylation Metabolism
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=mco1303391786

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-mco13033917862021-08-03T05:38:54Z Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases Alazizi, Adnan Animal Sciences Biochemistry Biomedical Research Pharmacology Toxicology Zebrafish Catechol O-methyltransferases COMT Methylation Metabolism The zebrafish is emerging as an important animal model for biomedical research. This study represents part of an effort to establish the zebrafish as a model for investigating the methylation pathway of drug metabolism. Two zebrafish catechol O-methyltransferases (COMTs), designated COMT-1 and COMT-2, were cloned, expressed, purified, and characterized. Substrate specificity of these two zebrafish COMTs were analyzed using a panel of catechol drugs including dobutamine, methyldopa, and isoproterenol, as well as endogenous catecholestrogens, catecholamines, and dopa. The expression of COMT-1 was examined from embryogenesis to maturity by employing both the conventional RT-PCR and RT-quantitative PCR. Data on the COMT-1 expression provided insights into the developmental expression of the COMT enzyme, which may serve as a basis for further investigation into its possible protective role against the adverse effects of catechol drugs and other xenobiotic catechols during the developmental process. 2011-06-15 English text University of Toledo Health Science Campus / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=mco1303391786 http://rave.ohiolink.edu/etdc/view?acc_num=mco1303391786 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Animal Sciences Biochemistry Biomedical Research Pharmacology Toxicology Zebrafish Catechol O-methyltransferases COMT Methylation Metabolism
spellingShingle	Animal Sciences Biochemistry Biomedical Research Pharmacology Toxicology Zebrafish Catechol O-methyltransferases COMT Methylation Metabolism Alazizi, Adnan Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases
author	Alazizi, Adnan
author_facet	Alazizi, Adnan
author_sort	Alazizi, Adnan
title	Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases
title_short	Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases
title_full	Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases
title_fullStr	Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases
title_full_unstemmed	Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases
title_sort	molecular cloning, expression, purification and characterization of the zebrafish catechol-o-methyltransferases
publisher	University of Toledo Health Science Campus / OhioLINK
publishDate	2011
url	http://rave.ohiolink.edu/etdc/view?acc_num=mco1303391786
work_keys_str_mv	AT alaziziadnan molecularcloningexpressionpurificationandcharacterizationofthezebrafishcatecholomethyltransferases
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Molecular Cloning, Expression, purification and Characterization of the Zebrafish Catechol-O-methyltransferases

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