Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)

Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)

Bibliographic Details
Main Author: Garapati, Chandrasekhar
Language:English
Published: University of Toledo Health Science Campus / OhioLINK 2014
Subjects:
Pharmaceuticals
Pharmacy Sciences
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514
id ndltd-OhioLink-oai-etd.ohiolink.edu-mco1404579514
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-mco14045795142021-08-03T06:25:31Z Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT) Garapati, Chandrasekhar Pharmaceuticals Pharmacy Sciences Antimicrobial therapy for sinusitis has been shown to reduce or eliminate pathologic bacteria associated with rhinosinusitis and improve the symptoms associated with the disease. However, the continuing rise in antibiotic resistance, the ongoing problem with patient compliance, and the intrinsic difficulty in eradication of biofilms complicates antibiotic therapy. The introduction of photodynamic antimicrobial therapy (PAT) using erythrosine, a photosensitizer, could eliminate the bacteria without inducing antibiotic resistance or even requiring daily dosing. In the present study, erythrosine nanoparticles were prepared using poly-lactic-co-glycolic acid (PLGA) and evaluated for their potential in PAT against Staphylococcus aureus bio¿lms. PLGA nanoparticles of erythrosine were prepared by nanoprecipitation technique. Erythrosine nanoparticles were characterized for size, zeta potential, morphology and in vitro release. Qualitative and quantitative uptake studies of erythrosine nanoparticles were carried out in S. aureus biofilms. Photodynamic inactivation of S. aureus biofilms in the presence of erythrosine nanoparticles was investigated by colony forming unit assay. Nanoprecipitation technique resulted in nanoparticles with a mean diameter of 385nm and zeta potential of -9.36 mV. Erythrosine was slowly released from nanoparticles over a period of 120 h. The qualitative study using flow cytometry showed the ability of S. aureus cells to internalize erythrosine nanoparticles. Moreover, erythrosine nanoparticles exhibited a significantly higher uptake and antimicrobial efficacy compared to pure drug in S. aureus biofilms. In conclusion, erythrosine-loaded PLGA nanoparticles can be a potential long term drug delivery system for PAT and are useful for the eradication of S.aureus biofilms. 2014-10-10 English text University of Toledo Health Science Campus / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514 http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Pharmaceuticals
Pharmacy Sciences
spellingShingle Pharmaceuticals
Pharmacy Sciences
Garapati, Chandrasekhar
Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)
author Garapati, Chandrasekhar
author_facet Garapati, Chandrasekhar
author_sort Garapati, Chandrasekhar
title Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)
title_short Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)
title_full Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)
title_fullStr Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)
title_full_unstemmed Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)
title_sort development and invitro characterization of erythrosine nanoparticles for chronic sinusitis using phtotdynamic therapy (pdt)
publisher University of Toledo Health Science Campus / OhioLINK
publishDate 2014
url http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514
work_keys_str_mv AT garapatichandrasekhar developmentandinvitrocharacterizationoferythrosinenanoparticlesforchronicsinusitisusingphtotdynamictherapypdt
_version_ 1719436360486486016

Similar Items