NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS

NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS

Bibliographic Details
Main Author: Nayar, Shweta
Language:English
Published: Miami University / OhioLINK 2013
Subjects:
Zoology
Neurosciences
Opiates
Prolactin
Corticosterone
HPA axis
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1382509698
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami13825096982021-08-03T06:20:07Z NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS Nayar, Shweta Zoology Neurosciences Opiates Prolactin Corticosterone HPA axis The role of N/OFQ in regulating the HPA axis under resting and stress conditions was determined using postpartum and virgin female N/OFQ wildtype and knockout mice. The interaction between prolactin (PRL) and the HPA axis was investigated using postpartum female mice housed with their pups, i.e. under hyperprolactinemic conditions and separated from their pups for 10 minutes or 24 hours, i.e. under normal prolactinemic conditions. Circulating levels of corticosterone (CORT) were used as a marker of HPA axis activation. Circulating CORT and PRL levels decreased in all postpartum females by 24 hours after delivery, but there was no difference between genotypes. Similarly, genotype had no effect on hypothalamic Corticotrophin Releasing Hormone (CRH) or choroid plexus PRL-R mRNA levels in postpartum females, indicating N/OFQ does not regulate the HPA axis under resting conditions. When postpartum animals were stressed, CORT levels increased, but there was no difference between genotypes. Hypothalamic CRH content was not affected by stress or genotype. Stress increased PRL mRNA levels, but this was also not different between the genotypes. These results indicate that N/OFQ is not necessary for activation of the HPA axis either under resting or stress conditions. Finally, studies using virgin female PRL wildtype and knockout mice indicate that PRL is not vital to the stress response. Taken together, these results demonstrate that N/OFQ does not regulate HPA axis activation under resting or stress conditions and that PRL does not play a critical role in the regulation of this axis during stress. While prolactinemic state influences the stress response, the response persists when PRL levels are normal, elevated or even absent. 2013-11-16 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1382509698 http://rave.ohiolink.edu/etdc/view?acc_num=miami1382509698 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Zoology
Neurosciences
Opiates
Prolactin
Corticosterone
HPA axis
spellingShingle Zoology
Neurosciences
Opiates
Prolactin
Corticosterone
HPA axis
Nayar, Shweta
NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS
author Nayar, Shweta
author_facet Nayar, Shweta
author_sort Nayar, Shweta
title NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS
title_short NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS
title_full NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS
title_fullStr NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS
title_full_unstemmed NOCICEPTIN/ORPHANIN FQ (N/OFQ) REGULATION OF THE STRESS RESPONSE: INTERACTION BETWEEN PROLACTIN AND THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS
title_sort nociceptin/orphanin fq (n/ofq) regulation of the stress response: interaction between prolactin and the hypothalamic-pituitary-adrenal (hpa) axis
publisher Miami University / OhioLINK
publishDate 2013
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1382509698
work_keys_str_mv AT nayarshweta nociceptinorphaninfqnofqregulationofthestressresponseinteractionbetweenprolactinandthehypothalamicpituitaryadrenalhpaaxis
_version_ 1719434960656400384

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