Effect of Aloe striata Inner Leaf Gel on Early Hyphal Development and Adhesion in Paecilomyces variotii, Fusarium oxysporum, and Fusarium solani

Bibliographic Details
Main Author:	Wada, Gloria Achibi
Language:	English
Published:	Miami University / OhioLINK 2016
Subjects:	Biology Microbiology Adhesion Paecilomyces variotii Fusarium oxysporum Fusarium solani Aloe striata
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=miami1459210755

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-miami14592107552021-08-03T06:35:05Z Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i> Wada, Gloria Achibi Biology Microbiology Adhesion Paecilomyces variotii Fusarium oxysporum Fusarium solani Aloe striata <p>Members of the <i>Fusarium solani</i> and <i>Fusarium oxysporum</i> species complexes are the most implicated etiologic agents in opportunistic fusarial infections in mammals while <i>Paecilomyces variotii</i> is one of the most frequently encountered <i>Paecilomyces</i> species in human infections. Prevention and treatment of these mycoses are problematic because available antimycotics are limited and often have toxic side effects. Popular folk medicines, such as the inner leaf gel from <i>Aloe</i> spp., are potential sources for non-toxic novel antimycotic compounds.To screen for antifungal properties of a non-domesticated <i>Aloe</i> species, <i>Aloe striata</i>, germination assays with homogenized 0.2 µm filtered <i>A. striata</i> inner leaf gel were performed against conidia of 3 strains each of <i>P. variotii</i>, <i>F. solani</i> and <i>F. oxysporum</i>. Although exposure to <i>A. striata</i> inner leaf gel caused only minimal inhibition of conidial germination for all strains, it caused visible hyphal aberrations characterized by increased hyphal diameters that lead to intervals of non-parallel hyphal cell walls as well as increased parental cell diameters. Adhesion assay results indicated that <i>A. striata </i>inner leaf gel induced hyphal aberrations significantly contribute to a decrease in the ability of 3 <i>P. variotii</i> strains to successfully remain adhered to microscope slides.</p><p>To isolate and identify the fractions of <i>A. striata</i> inner leaf gel responsible for hyphal aberrations, a combination of chromatographic techniques was used. A reverse phase high performance liquid chromatography (RP-HPLC) generated fraction, fraction A, demonstrated the most significant induction of hyphal aberrations. When fraction A was further separated, we identified fraction AIa as the portion of fraction A that caused the most significant hyphal aberration frequency increase in <i>P. variotii</i> ATCC 22319.</p><p>Our findings implicate <i>A. striata</i> inner leaf gel fraction AIa as the source of hyphal aberration frequency increases in <i>P. variotii</i>, <i>F. oxysporum</i>, and <i>F. solani</i>. Since hyphal aberrations contribute to a decrease in adhesion frequency, an important fungal virulence factor, we have identified <i>A. striata</i> inner leaf gel fraction AIa as a mixture of compounds with novel antimycotic properties that could potentially be used to combat adhesion and help reduce and/or prevent fungal colonization of hosts and/or substrates.</p> 2016-03-29 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1459210755 http://rave.ohiolink.edu/etdc/view?acc_num=miami1459210755 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biology Microbiology Adhesion Paecilomyces variotii Fusarium oxysporum Fusarium solani Aloe striata
spellingShingle	Biology Microbiology Adhesion Paecilomyces variotii Fusarium oxysporum Fusarium solani Aloe striata Wada, Gloria Achibi Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>
author	Wada, Gloria Achibi
author_facet	Wada, Gloria Achibi
author_sort	Wada, Gloria Achibi
title	Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>
title_short	Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>
title_full	Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>
title_fullStr	Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>
title_full_unstemmed	Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>
title_sort	effect of <i>aloe striata</i> inner leaf gel on early hyphal development and adhesion in <i>paecilomyces variotii</i>, <i>fusarium oxysporum</i>, and <i>fusarium solani</i>
publisher	Miami University / OhioLINK
publishDate	2016
url	http://rave.ohiolink.edu/etdc/view?acc_num=miami1459210755
work_keys_str_mv	AT wadagloriaachibi effectofialoestriataiinnerleafgelonearlyhyphaldevelopmentandadhesioninipaecilomycesvariotiiiifusariumoxysporumiandifusariumsolanii
_version_	1719439590375292928

Effect of <i>Aloe striata</i> Inner Leaf Gel on Early Hyphal Development and Adhesion in <i>Paecilomyces variotii</i>, <i>Fusarium oxysporum</i>, and <i>Fusarium solani</i>

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